Show simple item record

dc.contributor.authorPym, Edward CGen
dc.contributor.authorSouthall, Tonyen
dc.contributor.authorMee, Christopher Jen
dc.contributor.authorBrand, Andreaen
dc.contributor.authorBaines, Richard Aen
dc.date.accessioned2011-06-16T16:46:19Z
dc.date.available2011-06-16T16:46:19Z
dc.date.issued2006-11-16en
dc.identifier.citationNeural Development 2006, 1:3
dc.identifier.issn1749-8104
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/238058
dc.description.abstractAbstract Background While developmental processes such as axon pathfinding and synapse formation have been characterized in detail, comparatively less is known of the intrinsic developmental mechanisms that regulate transcription of ion channel genes in embryonic neurons. Early decisions, including motoneuron axon targeting, are orchestrated by a cohort of transcription factors that act together in a combinatorial manner. These transcription factors include Even-skipped (Eve), islet and Lim3. The perdurance of these factors in late embryonic neurons is, however, indicative that they might also regulate additional aspects of neuron development, including the acquisition of electrical properties. Results To test the hypothesis that a combinatorial code transcription factor is also able to influence the acquisition of electrical properties in embryonic neurons we utilized the molecular genetics of Drosophila to manipulate the expression of Eve in identified motoneurons. We show that increasing expression of this transcription factor, in two Eve-positive motoneurons (aCC and RP2), is indeed sufficient to affect the electrical properties of these neurons in early first instar larvae. Specifically, we observed a decrease in both the fast K+ conductance (IKfast) and amplitude of quantal cholinergic synaptic input. We used charybdotoxin to pharmacologically separate the individual components of IKfast to show that increased Eve specifically down regulates the Slowpoke (a BK Ca2+-gated potassium channel), but not Shal, component of this current. Identification of target genes for Eve, using DNA adenine methyltransferase identification, revealed strong binding sites in slowpoke and nAcRα-96Aa (a nicotinic acetylcholine receptor subunit). Verification using real-time PCR shows that pan-neuronal expression of eve is sufficient to repress transcripts for both slo and nAcRα-96Aa. Conclusion Taken together, our findings demonstrate, for the first time, that Eve is sufficient to regulate both voltage- and ligand-gated currents in motoneurons, extending its known repertoire of action beyond its already characterized role in axon guidance. Our data are also consistent with a common developmental program that utilizes a defined set of transcription factors to determine both morphological and functional neuronal properties.
dc.languageEnglishen
dc.language.isoen
dc.titleThe homeobox transcription factor Even-skipped regulates acquisition of electrical properties in Drosophilaneuronsen
dc.typeArticle
dc.date.updated2011-06-16T16:46:19Z
dc.rights.holderPym et al.; licensee BioMed Central Ltd.
prism.publicationDate2006en
dcterms.dateAccepted2006-11-16en
rioxxterms.versionofrecord10.1186/1749-8104-1-3en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2006-11-16en
dc.contributor.orcidBrand, Andrea [0000-0002-2089-6954]
dc.identifier.eissn1749-8104
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (G0300072)


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record