Show simple item record

dc.contributor.authorGorodkin, Jan
dc.contributor.authorCirera, Susanna
dc.contributor.authorHedegaard, Jakob
dc.contributor.authorGilchrist, Michael J
dc.contributor.authorPanitz, Frank
dc.contributor.authorJørgensen, Claus
dc.contributor.authorScheibye-Knudsen, Karsten
dc.contributor.authorArvin, Troels
dc.contributor.authorLumholdt, Steen
dc.contributor.authorSawera, Milena
dc.contributor.authorGreen, Trine
dc.contributor.authorNielsen, Bente J
dc.contributor.authorHavgaard, Jakob H
dc.contributor.authorRosenkilde, Carina
dc.contributor.authorWang, Jun
dc.contributor.authorLi, Heng
dc.contributor.authorLi, Ruiqiang
dc.contributor.authorLiu, Bin
dc.contributor.authorHu, Songnian
dc.contributor.authorDong, Wei
dc.contributor.authorLi, Wei
dc.contributor.authorYu, Jun
dc.contributor.authorWang, Jian
dc.contributor.authorStaefeldt, Hans-Henrik
dc.contributor.authorWernersson, Rasmus
dc.contributor.authorMadsen, Lone B
dc.contributor.authorThomsen, Bo
dc.contributor.authorHornshøj, Henrik
dc.contributor.authorBujie, Zhan
dc.contributor.authorWang, Xuegang
dc.contributor.authorWang, Xuefei
dc.contributor.authorBolund, Lars
dc.contributor.authorBrunak, Søren
dc.contributor.authorYang, Huanming
dc.contributor.authorBendixen, Christian
dc.contributor.authorFredholm, Merete
dc.date.accessioned2011-06-17T14:30:00Z
dc.date.available2011-06-17T14:30:00Z
dc.date.issued2007
dc.identifierhttp://dx.doi.org/10.1186/gb-2007-8-4-r45
dc.identifier.issn1474-760X
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/238226
dc.descriptionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.
dc.description.abstractBACKGROUND: Knowledge of the structure of gene expression is essential for mammalian transcriptomics research. We analyzed a collection of more than one million porcine expressed sequence tags (ESTs), of which two-thirds were generated in the Sino-Danish Pig Genome Project and one-third are from public databases. The Sino-Danish ESTs were generated from one normalized and 97 non-normalized cDNA libraries representing 35 different tissues and three developmental stages. RESULTS: Using the Distiller package, the ESTs were assembled to roughly 48,000 contigs and 73,000 singletons, of which approximately 25% have a high confidence match to UniProt. Approximately 6,000 new porcine gene clusters were identified. Expression analysis based on the non-normalized libraries resulted in the following findings. The distribution of cluster sizes is scaling invariant. Brain and testes are among the tissues with the greatest number of different expressed genes, whereas tissues with more specialized function, such as developing liver, have fewer expressed genes. There are at least 65 high confidence housekeeping gene candidates and 876 cDNA library-specific gene candidates. We identified differential expression of genes between different tissues, in particular brain/spinal cord, and found patterns of correlation between genes that share expression in pairs of libraries. Finally, there was remarkable agreement in expression between specialized tissues according to Gene Ontology categories. CONCLUSION: This EST collection, the largest to date in pig, represents an essential resource for annotation, comparative genomics, assembly of the pig genome sequence, and further porcine transcription studies.
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll Rights Reserved
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/
dc.titlePorcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
dc.typeArticle
dc.date.updated2011-06-17T14:30:00Z
dc.description.versionPublished version
dc.rights.holderGorodkin et al.; licensee BioMed Central Ltd.
prism.publicationNameGenome Biol
pubs.declined2017-10-11T13:54:29.275+0100
dcterms.dateAccepted2007-04-02
rioxxterms.versionofrecord10.1186/gb-2007-8-4-r45
dc.identifier.eissn1474-760X


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record