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dc.contributor.authorFranz, Henrietteen
dc.contributor.authorUllmann, Claudiaen
dc.contributor.authorBecker, Alberten
dc.contributor.authorRyan, Margareten
dc.contributor.authorBahn, Sabineen
dc.contributor.authorArendt, Thomasen
dc.contributor.authorSimon, Matthiasen
dc.contributor.authorPaabo, Svanteen
dc.contributor.authorKhaitovich, Philippen
dc.date.accessioned2011-06-17T14:31:55Z
dc.date.available2011-06-17T14:31:55Z
dc.date.issued2005-12-30en
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/238246
dc.description.abstractAbstract Background Numerous studies have employed microarray techniques to study changes in gene expression in connection with human disease, aging and evolution. The vast majority of human samples available for research are obtained from deceased individuals. This raises questions about how well gene expression patterns in such samples reflect those of living individuals. Results Here, we compare gene expression patterns in two human brain regions in postmortem samples and in material collected during surgical intervention. We find that death induces significant expression changes in more than 10% of all expressed genes. These changes are non-randomly distributed with respect to their function. Moreover, we observe similar expression changes due to death in two distinct brain regions. Consequently, the pattern of gene expression differences between the two brain regions is largely unaffected by death, although the magnitude of differences is reduced by 50% in postmortem samples. Furthermore, death-induced changes do not contribute significantly to gene expression variation among postmortem human brain samples. Conclusion We conclude that postmortem human brain samples are suitable for investigating gene expression patterns in humans, but that caution is warranted in interpreting results for individual genes.
dc.languageEnglishen
dc.language.isoen
dc.titleSystematic analysis of gene expression in human brains before and after deathen
dc.typeArticle
dc.date.updated2011-06-17T14:31:56Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderFranz et al.; licensee BioMed Central Ltd.
prism.publicationDate2005en
dcterms.dateAccepted2005-12-06en
rioxxterms.versionofrecord10.1186/gb-2005-6-13-r112en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2005-12-30en
rioxxterms.typeJournal Article/Reviewen


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