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dc.contributor.authorGordon, Katrina Een
dc.contributor.authorBinas, Berten
dc.contributor.authorChapman, Rachel Sen
dc.contributor.authorKurian, Kathreena Men
dc.contributor.authorClarkson, Richard WEen
dc.contributor.authorClark, A Johnen
dc.contributor.authorLane, E Birgitteen
dc.contributor.authorWatson, Christineen
dc.date.accessioned2011-06-17T14:34:26Z
dc.date.available2011-06-17T14:34:26Z
dc.date.issued2000-03-07en
dc.identifier.issn1465-5411
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/238283
dc.description.abstractAbstract Background This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine β-lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag). Results KIM-2 cells have a characteristic luminal epithelial cell morphology and a stable, nontransformed phenotype at the semipermissive temperature of 37°C. In contrast, at the permissive temperature of 33°C the cells have an elongated spindle-like morphology and become transformed after prolonged culture. Differentiation of KIM-2 cells at 37°C, in response to lactogenic hormones, results in the formation of polarized dome-like structures with tight junctions. This is accompanied by expression of the milk protein genes that encode β-casein and whey acidic protein (WAP), and activation of the prolactin signalling molecule, signal transducer and activator of transcription (STAT)5. Fully differentiated KIM-2 cultures at 37°C become dependent on lactogenic hormones for survival and undergo extensive apoptosis upon hormone withdrawal, as indicated by nuclear morphology and flow cytometric analysis. KIM-2 cells can be genetically modified by stable transfection and clonal lines isolated that retain the characteristics of untransfected cells. Conclusion KIM-2 cells are a valuable addition, therefore, to currently available lines of mammary epithelial cells. Their capacity for extensive differentiation in the absence of exogenously added basement membrane, and ability to undergo apoptosis in response to physiological signals will provide an invaluable model system for the study of signal transduction pathways and transcriptional regulatory mechanisms that control differentiation and involution in the mammary gland.
dc.languageEnglishen
dc.language.isoen
dc.titleA novel cell culture model for studying differentiation and apoptosis in the mouse mammary glanden
dc.typeArticle
dc.date.updated2011-06-17T14:34:26Z
dc.rights.holderGordon et al.; licensee BioMed Central Ltd.
prism.publicationDate2000en
dcterms.dateAccepted2000-02-08en
rioxxterms.versionofrecord10.1186/bcr57en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2000-03-07en
dc.contributor.orcidWatson, Christine [0000-0002-8548-5902]
dc.identifier.eissn1465-542X
rioxxterms.typeJournal Article/Reviewen


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