The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor
Smith, J. C.
Richards, B. M.
Barnard, E. A.
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Sumikawa, K., Houghton, M., Smith, J. C., Bell, L., Richards, B. M., & Barnard, E. A. (1982). The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
The published version of this article is available at Oxford Journals in Nucleic Acids Research at http://nar.oxfordjournals.org/content/10/19/5809.full.pdf+html
A rare cDNA coding for most of the α subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane. When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with a subunit mRNA extracted from cat muscle.
This record's URL: http://www.dspace.cam.ac.uk/handle/1810/241098