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dc.contributor.authorShepherd, Jenniferen
dc.contributor.authorGhose, Sen
dc.contributor.authorKew, SJen
dc.contributor.authorMoavenian, Aen
dc.contributor.authorBest, Serenaen
dc.contributor.authorCameron, Ruthen
dc.date.accessioned2013-02-14T12:41:10Z
dc.date.available2013-02-14T12:41:10Z
dc.date.issued2013-01-01en
dc.identifier.issn1549-3296
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/244252
dc.description.abstractPorous collagen–glycosaminoglycan structures are bioactive and exhibit a pore architecture favorable for both cellular infiltration and attachment; however, their inferior mechanical properties limit use, particularly in load-bearing situations. Reinforcement with collagen fibers may be a feasible route for enhancing the mechanical characteristics of these materials, providing potential for composites used for the repair and regeneration of soft tissue such as tendon, ligaments, and cartilage. Therefore, this study investigates the reinforcement of collagen–chondroitin-6-sulfate (C6S) porous structures with bundles of extruded, reconstituted type I collagen fibers. Fiber bundles were produced through extrusion and then, where applicable, crosslinked using a solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide. Fibers were then submerged in the collagen–C6S matrix slurry before being lyophilized. A second 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide crosslinking process was then applied to the composite material before a secondary lyophilization cycle. Where bundles had been previously crosslinked, composites withstood a load of approximately 60 N before failure, the reinforcing fibers remained dense and a favorable matrix pore structure resulted, with good interaction between fiber and matrix. Fibers that had not been crosslinked before lyophilization showed significant internal porosity and a channel existed between them and the matrix. Mechanical properties were significantly reduced, but the additional porosity could prove favorable for cell migration and has potential for directing aligned tissue growth.
dc.titleEffect of fiber crosslinking on collagen-fiber reinforced collagen-chondroitin-6-sulfate materials for regenerating load-bearing soft tissuesen
dc.typeArticle
dc.description.versionThis is the pre-peer reviewed version of the following article: J.H. Shepherd, S. Ghose, A. Moavenian, S.J. Kew, S.M. Best and R.E. Cameron. “Effect of fibre Cross-linking on Collagen-fibre reinforced Collagen-chondroitin-6-sulphate materials for regenerating load-bearing soft tissues”. Journal of Biomedical Materials Research: Part A, 2013;101(1):176-84., which has been published in final form at http://dx.doi.org/10.1002/jbm.a.34317.en
prism.publicationDate2013en
prism.startingPage176
prism.volume101Aen
pubs.end-page184
dcterms.dateAccepted2012-05-15en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2013-01-01en
dc.contributor.orcidBest, Serena [0000-0001-7866-8607]
dc.contributor.orcidCameron, Ruth [0000-0003-1573-4923]
dc.identifier.eissn1552-4965
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (DT/F006977/1)


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