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dc.contributor.authorWang, Laura Yen
dc.contributor.authorSummerhill, Keithen
dc.contributor.authorRodriguez-Canas, Carmenen
dc.contributor.authorMather, Ianen
dc.contributor.authorPatel, Pinalen
dc.contributor.authorEiden, Michaelen
dc.contributor.authorYoung, Stephenen
dc.contributor.authorForouhi, Nitaen
dc.contributor.authorKoulman, Alberten
dc.date.accessioned2013-06-13T19:03:34Z
dc.date.available2013-06-13T19:03:34Z
dc.date.issued2013-04-25en
dc.identifier.issn1756-994X
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/244678
dc.description.abstractAbstract A fully automated, high-throughput method was developed to profile the fatty acids of phospholipids from human plasma samples for application to a large epidemiological sample set (n > 25,000). We report here on the data obtained for the quality-control materials used with the first 860 batches, and the validation process used. The method consists of two robotic systems combined with gas chromatography, performing lipid extraction, phospholipid isolation, hydrolysis and derivatization to fatty-acid methyl esters, and on-line analysis. This is the first report showing that fatty-acid profiling is an achievable strategy for metabolic phenotyping in very large epidemiological and genetic studies.
dc.languageEnglishen
dc.language.isoen
dc.titleDevelopment and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studiesen
dc.typeArticle
dc.date.updated2013-06-13T19:03:34Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderLaura Wang et al.; licensee BioMed Central Ltd.
prism.publicationDate2013en
dcterms.dateAccepted2013-04-25en
rioxxterms.versionofrecord10.1186/gm443en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2013-04-25en
dc.contributor.orcidForouhi, Nita [0000-0002-5041-248X]
dc.contributor.orcidKoulman, Albert [0000-0001-9998-051X]
dc.identifier.eissn1756-994X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_UU_12015/5)
pubs.funder-project-idMedical Research Council (MC_U106179471)


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