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Analysis of the hydration water around bovine serum albumin using terahertz coherent synchrotron radiation.


Type

Article

Change log

Authors

Bye, Jordan W 
Meliga, Stefano 
Ferachou, Denis 
Cinque, Gianfelice 
Zeitler, J Axel 

Abstract

Terahertz spectroscopy was used to study the absorption of bovine serum albumin (BSA) in water. The Diamond Light Source operating in a low alpha mode generated coherent synchrotron radiation that covered a useable spectral bandwidth of 0.3-3.3 THz (10-110 cm(-1)). As the BSA concentration was raised, there was a nonlinear change in absorption inconsistent with Beer's law. At low BSA concentrations (0-1 mM), the absorption remained constant or rose slightly. Above a concentration of 1 mM BSA, a steady decrease in absorption was observed, which was followed by a plateau that started at 2.5 mM. Using a overlapping hydration layer model, the hydration layer was estimated to extend 15 Å from the protein. Calculation of the corrected absorption coefficient (αcorr) for the water around BSA by subtracting the excluded volume of the protein provides an alternative approach to studying the hydration layer that provides evidence for complexity in the population of water around BSA.

Description

Keywords

Animals, Cattle, Models, Molecular, Serum Albumin, Bovine, Terahertz Spectroscopy, Water

Journal Title

J Phys Chem A

Conference Name

Journal ISSN

1089-5639
1520-5215

Volume Title

Publisher

American Chemical Society (ACS)