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Mammary stem cells have myoepithelial cell properties.


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Article

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Authors

Prater, Michael D 
Petit, Valérie 
Alasdair Russell, I 
Giraddi, Rajshekhar R 
Shehata, Mona 

Abstract

Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin-myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU). We demonstrate that myoepithelial cells, flow-sorted using two independent myoepithelial-specific reporter strategies, have MRU capacity. Using an inducible lineage-tracing approach we follow the progeny of myoepithelial cells that express α-smooth muscle actin and show that they function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy.

Description

Keywords

Actins, Animals, Benzamides, Cell Proliferation, Cells, Cultured, Dioxoles, Epithelial Cells, Female, Flow Cytometry, Gene Expression Profiling, Green Fluorescent Proteins, Interleukin Receptor Common gamma Subunit, Mammary Glands, Animal, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred NOD, Mice, SCID, Mice, Transgenic, Microscopy, Fluorescence, Myocytes, Smooth Muscle, Oligonucleotide Array Sequence Analysis, Receptors, Transforming Growth Factor beta, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells

Journal Title

Nat Cell Biol

Conference Name

Journal ISSN

1465-7392
1476-4679

Volume Title

Publisher

Springer Science and Business Media LLC

Rights

DSpace@Cambridge license
Sponsorship
Breast Cancer Now (None)
This work was funded by Cancer Research UK, Breast Cancer Campaign, the University of Cambridge, Hutchison Whampoa Limited, La Ligue Nationale Contre le Cancer (Equipe Labelisée 2013) and a grant from Agence Nationale de la Recherche ANR- 08-BLAN-0078-01 to M.A.G.