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Chemical biology of genomic DNA: minimizing PCR bias.


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Authors

McInroy, Gordon R 
Raiber, Eun-Ang 
Balasubramanian, Shankar  ORCID logo  https://orcid.org/0000-0002-0281-5815

Abstract

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

Description

Keywords

Aldehydes, Biotinylation, DNA, DNA Adducts, DNA Probes, Hydrogen-Ion Concentration, Oxidation-Reduction, Real-Time Polymerase Chain Reaction

Journal Title

Chem Commun (Camb)

Conference Name

Journal ISSN

1359-7345
1364-548X

Volume Title

50

Publisher

Royal Society of Chemistry (RSC)
Sponsorship
Engineering and Physical Sciences Research Council (EP/K039520/1)
Wellcome Trust (099232/Z/12/Z)
GRM is supported by Trinity College and Herchel Smith studentships. EAR is a Herchel Smith Fellow. The SB lab is supported by core funding from Cancer Research UK. SB is a Wellcome Trust Senior Investigator.