Chemical biology of genomic DNA: minimizing PCR bias.
Change log
Authors
Abstract
The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
Description
Keywords
Aldehydes, Biotinylation, DNA, DNA Adducts, DNA Probes, Hydrogen-Ion Concentration, Oxidation-Reduction, Real-Time Polymerase Chain Reaction
Journal Title
Chem Commun (Camb)
Conference Name
Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
50
Publisher
Royal Society of Chemistry (RSC)
Publisher DOI
Sponsorship
Engineering and Physical Sciences Research Council (EP/K039520/1)
Wellcome Trust (099232/Z/12/Z)
Wellcome Trust (099232/Z/12/Z)
GRM is supported by Trinity College and Herchel Smith studentships. EAR is a Herchel Smith Fellow. The SB lab is supported by core funding from Cancer Research UK. SB is a Wellcome Trust Senior Investigator.