BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo
Graham, Sarah JL
Wicher, Krzysztof B
Nature Publishing Group
Article number 5667
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Graham, S. J., Wicher, K. B., Jedrusik, A., Guo, G., Herath, W., Robson, P., & Zernicka-Goetz, M. (2014). BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo. Nature Communications, 5 (Article number 5667)https://doi.org/10.1038/ncomms6667
Pre-implantation development requires the specification and organization of embryonic and extra-embryonic lineages. The separation of these lineages takes place when asymmetric divisions generate inside and outside cells that differ in polarity, position and fate. Here we assess the global transcriptional identities of these precursor cells to gain insight into the molecular mechanisms regulating lineage segregation,. Unexpectedly, this reveals that complementary components of the BMP signalling pathway are already differentially expressed after the first wave of asymmetric divisions. We investigate the role of BMP signalling by expressing dominant negative forms of Smad4 and Bmpr2, by down-regulating the pathway using RNAi against BMP ligands and by applying three different BMP inhibitors at distinct stages. This reveals that BMP signalling regulates the correct development of both extra-embryonic lineages, primitive endoderm and trophectoderm, but not the embryonic lineage, prior to implantation. Together these findings indicate multiple roles of BMP signalling in the early mouse embryo.
embryo, mouse, BMP signalling, development, pluripotency, differentiation
This work was supported by a Wellcome Trust programme grant to MZG and an Agency for Science, Technology and Research (A*Star) core research budget to PR.
Wellcome Trust (098287/Z/12/Z)
External DOI: https://doi.org/10.1038/ncomms6667
This record's URL: https://www.repository.cam.ac.uk/handle/1810/246215