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dc.contributor.authorGetgood, Alanen
dc.contributor.authorHenson, Francesen
dc.contributor.authorSkelton, Carrieen
dc.contributor.authorBrooks, Rogeren
dc.contributor.authorGuehring, Hansen
dc.contributor.authorFortier, Lisa Aen
dc.contributor.authorRushton, Neilen
dc.date.accessioned2014-10-20T14:15:05Z
dc.date.available2014-10-20T14:15:05Z
dc.date.issued2014-09-26en
dc.identifier.citation(2014) Journal of Experimental Orthopaedics 1: 13en
dc.identifier.issn2197-1153
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/246223
dc.description.abstractBackground The aim of this study was to investigate the effect of combining rhFGF18 or BMP-7 with a biphasic collagen/GAG osteochondral scaffold (Chondromimetic) on the repair of osteochondral defects in sheep. Methods Osteochondral defects (5.8x6mm) were created in the medial femoral condyle (MFC) and the lateral trochlea sulcus (LTS) of the stifle joint of 24 female sheep. Sheep were randomly assigned to four groups (n = 6); 1) empty defect, 2) scaffold only, 3) scaffold + rhFGF-18 (30 μg) and 4) scaffold + BMP-7 (100 μg). At 6 months the defects underwent non-destructive mechanical testing, gross assessment of repair tissue (ICRS score) and histological analysis (Modified O’Driscoll score). Results ICRS repair score: Defects treated with scaffold + rhFGF18 (mean 9.83, 95% CI 8.43-11.23) and scaffold + BMP-7 (10, 9.06-10.94) in the MFC had significantly improved ICRS scores compared to empty defects (4.2, 0–8.80) (p = 0.002). Mechanical properties: BMP-7 treated defects (mean 64.35, 95% CI 56.88-71.82) were significantly less stiff than both the rhFGF18 (mean 84.1, 95% CI 76.8-91.4) and empty defects in the LTS, compared to both contralateral limb (p = 0.003), and the perilesional articular cartilage (p < 0.001). Histology: A statistically significant improvement in the modified O’Driscoll score was observed in the rhFGF18 treated group (mean 16.83, 95% CI 13.65-20.61) compared to the empty defects (mean 9, 95% CI 4.88-13.12) (p = 0.039) in the MFC. Excellent tissue fill, lateral integration and proteoglycan staining was observed. Only the rhFGF18 defects showed pericellular type VI collagen staining with positive type II collagen and reduced positive type I collagen staining. The majority of defects in the control and BMP-7 groups demonstrated fibrocartilagenous repair tissue. Conclusion Statistically significant improvements in gross repair, mechanical properties and histological score were found over empty defects when Chondromimetic was combined with rhFGF18. These results suggest that rhFGF18 may play a significant role in articular cartilage repair applications.
dc.languageen_USen
dc.language.isoen_USen
dc.publisherSpringer
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.titleOsteochondral tissue engineering using a biphasic collagen/GAG scaffold containing rhFGF18 or BMP7 in an ovine modelen
dc.typeArticle
dc.description.versionThis is the final published version. It is published by Springer in the Journal of Experimental Orthopaedics here: http://www.jeo-esska.com/content/1/1/13.en
prism.publicationDate2014en
prism.publicationNameJournal of Experimental Orthopaedicsen
prism.startingPage13
prism.volume1en
dc.rioxxterms.funderNational Institute for Health Research
dcterms.dateAccepted2014-08-20en
rioxxterms.versionofrecord10.1186/s40634-014-0013-xen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-09-26en
dc.contributor.orcidHenson, Frances [0000-0002-3518-1492]
dc.identifier.eissn2197-1153
rioxxterms.typeJournal Article/Reviewen


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales