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dc.contributor.authorWu, Chunlingen
dc.contributor.authorGoodall, Janeen
dc.contributor.authorBusch, Roberten
dc.contributor.authorGaston, Hillen
dc.date.accessioned2014-11-06T12:32:57Z
dc.date.available2014-11-06T12:32:57Z
dc.date.issued2014-08-12en
dc.identifier.citationClinical and Experimental Immunology Volume 179, Issue 3, pages 378–391, March 2015. DOI: 10.1111/cei.12434en
dc.identifier.issn0009-9104
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/246301
dc.description.abstractExpression of the adhesion molecule, CD146/MCAM/MelCAM, on T cells has been associated with recent activation, memory subsets, and Th17 effector function, and is elevated in inflammatory arthritis. Th17 cells have been implicated in the pathogenesis of rheumatoid arthritis (RA) and spondyloarthritides (SpA). Here, we compared the expression of CD146 on CD4^+ T cells between healthy donors (HD) and patients with RA and SpA (ankylosing spondylitis [AS] or psoriatic arthritis [PsA]) and examined correlations with surface markers and cytokine secretion. Peripheral blood mononuclear cells (PBMC) were obtained from patients and controls, and synovial fluid mononuclear cells (SFMC) from patients. Cytokine production (elicited by PMA/ionomycin) and surface phenotypes were evaluated by flow cytometry. CD146^+CD4^+ and IL-17^+CD4^+ T cell frequencies were increased in PBMC of PsA patients, compared with HD, and in SFMC compared with PBMC. CD146^+CD4^+ T cells were enriched for secretion of IL-17 (alone or with IL-22 or IFN-γ) and for some putative Th17-associated surface markers (CD161 and CCR6), but not others (CD26 and IL23 receptor). CD4^+ T cells producing IL-22 or IFN-γ without IL-17 were also present in the CD146^+ subset, although their enrichment was less marked. Moreover, a majority of cells secreting these cytokines lacked CD146. Thus, CD146 is not a sensitive or specific marker of Th17 cells, but rather correlates with heterogeneous cytokine secretion by subsets of CD4^+ helper T cells.
dc.languageEnglishen
dc.language.isoenen
dc.publisherWiley
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleRelationship of CD146 expression to secretion of interleukin-17, interleukin-22, and interferon-γ by CD4^+ T cells in patients with inflammatory arthritisen
dc.typeArticle
dc.description.versionThis is the final version. It was first published by Wiley at http://onlinelibrary.wiley.com/doi/10.1111/cei.12434/abstract.en
prism.endingPage391
prism.publicationDate2014en
prism.publicationNameClinical and Experimental Immunologyen
prism.startingPage378
prism.volume179en
dc.rioxxterms.funderNational Institute for Health Research
dcterms.dateAccepted2014-08-07en
rioxxterms.versionofrecord10.1111/cei.12434en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-08-12en
dc.contributor.orcidGoodall, Jane [0000-0002-3761-161X]
dc.contributor.orcidGaston, Hill [0000-0002-5789-5111]
dc.identifier.eissn1365-2249
rioxxterms.typeJournal Article/Reviewen


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales