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dc.contributor.authorChen, Luen
dc.contributor.authorKostadima, Myrtoen
dc.contributor.authorMartens, Joost HAen
dc.contributor.authorCanu, Giovannien
dc.contributor.authorGarcia, Sara Pen
dc.contributor.authorTurro Bassols, Ernesten
dc.contributor.authorDownes, Kateen
dc.contributor.authorMacaulay, Iain Cen
dc.contributor.authorBielczyk-Maczynska, Ewaen
dc.contributor.authorCoe, Sophiaen
dc.contributor.authorFarrow, Samanthaen
dc.contributor.authorPoudel, Pawanen
dc.contributor.authorBurden, Francesen
dc.contributor.authorJansen, Sjoert BGen
dc.contributor.authorAstle, Williamen
dc.contributor.authorAttwood, Antonyen
dc.contributor.authorBariana, Tadbiren
dc.contributor.authorde, Bono Bernarden
dc.contributor.authorBreschi, Alessandraen
dc.contributor.authorChambers, John Cen
dc.contributor.authorBRIDGE, Consortiumen
dc.contributor.authorChoudry, Fizzah Aen
dc.contributor.authorClarke, Lauraen
dc.contributor.authorCoupland, Paulen
dc.contributor.authorvan, der Ent Martijnen
dc.contributor.authorErber, Wendy Nen
dc.contributor.authorJansen, Joop Hen
dc.contributor.authorFavier, Rémien
dc.contributor.authorFenech, Matthew Een
dc.contributor.authorFoad, Nicolaen
dc.contributor.authorFreson, Kathleenen
dc.contributor.authorvan, Geet Chrisen
dc.contributor.authorGomez, Keithen
dc.contributor.authorGuigo, Rodericen
dc.contributor.authorHampshire, Danielen
dc.contributor.authorKelly, Anne Men
dc.contributor.authorKerstens, Hindrik HDen
dc.contributor.authorKooner, Jaspal Sen
dc.contributor.authorLaffan, Michaelen
dc.contributor.authorLentaigne, Claireen
dc.contributor.authorLabalette, Charlotteen
dc.contributor.authorMartin, Tiphaineen
dc.contributor.authorMeacham, Stuarten
dc.contributor.authorMumford, Andrewen
dc.contributor.authorNürnberg, Sylviaen
dc.contributor.authorPalumbo, Emilioen
dc.contributor.authorvan, der Reijden Bert Aen
dc.contributor.authorRichardson, Daviden
dc.contributor.authorSammut, Stephenen
dc.contributor.authorSlodkowicz, Gregen
dc.contributor.authorTamuri, Asif Uen
dc.contributor.authorVasquez, Louellaen
dc.contributor.authorVoss, Katrinen
dc.contributor.authorWatt, Stephenen
dc.contributor.authorWestbury, Sarahen
dc.contributor.authorFlicek, Paulen
dc.contributor.authorLoos, Remcoen
dc.contributor.authorGoldman, Nicken
dc.contributor.authorBertone, Paulen
dc.contributor.authorRead, Randyen
dc.contributor.authorRichardson, Sylviaen
dc.contributor.authorCvejic, Anaen
dc.contributor.authorSoranzo, Nicoleen
dc.contributor.authorOuwehand, Willemen
dc.contributor.authorStunnenberg, Hendrik Gen
dc.contributor.authorFrontini, Mattiaen
dc.contributor.authorRendon Restrepo, Augustoen
dc.date.accessioned2014-11-12T12:07:22Z
dc.date.available2014-11-12T12:07:22Z
dc.date.issued2014-09-26en
dc.identifier.citation(2014) Science 345(6204): 1251033en
dc.identifier.issn0036-8075
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/246350
dc.description.abstractBlood cells derive from hematopoietic stem cells through stepwise fating events. To characterize gene expression programs driving lineage choice we sequenced RNA from eight primary human hematopoietic progenitor populations representing the major myeloid commitment stages and the main lymphoid stage. We identify extensive cell-type specific expression changes: 6,711 genes and 10,724 transcripts, enriched in non-protein coding elements at early stages of differentiation. In addition, we discovered 7,881 novel splice junctions and 2,301 differentially used alternative splicing events, enriched in genes involved in regulatory processes. We demonstrate experimentally cell specific isoform usage, identifying NFIB as a regulator of megakaryocyte maturation − the platelet precursor. Our data highlight the complexity of fating events in closely related progenitor populations, the understanding of which is essential for the advancement of transplantation and regenerative medicine.
dc.languageen_USen
dc.language.isoen_USen
dc.publisherAAAS
dc.titleTranscriptional diversity during lineage commitment of human blood progenitorsen
dc.typeArticle
dc.description.versionThis is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on 26/9/14 in volume 345, number 6204, DOI: 10.1126/science.1251033. This version will be under embargo until the 26th of March 2015.en
prism.endingPage125103310
prism.publicationDate2014en
prism.publicationNameScienceen
prism.startingPage12510331
prism.volume345en
dc.rioxxterms.funderEuropean Commission; National Institute for Health Research; British Heart Foundation; NHS Blood and Transplant; Wellcome Trust; Cancer Research UK; Imperial College; Medical Research Council; British Society of Haematology/NHS Blood and Transplant; EMBL; FWO-Vlaanderen
dc.rioxxterms.projectidEC: FP7 BLUEPRINT (HEALTH-F5-2011-282510), NETSIM, EPIGENESYS (257082); BHF: RP-PG-0310-1002, RG/09/12/28096, FS/12/27/29405; WT: WT098051, WT091310, 084183/Z/07/Z, 082961/Z/07/Z, 095908; CRUK: C45041/A14953; FWO-Vlaanderen: G.0B17.13N
dcterms.dateAccepted2014-08-28en
rioxxterms.versionofrecord10.1126/science.1251033en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-09-26en
dc.contributor.orcidTurro Bassols, Ernest [0000-0002-1820-6563]
dc.contributor.orcidDownes, Kate [0000-0003-0366-1579]
dc.contributor.orcidAstle, William [0000-0001-8866-6672]
dc.contributor.orcidSammut, Stephen [0000-0003-4472-904X]
dc.contributor.orcidBertone, Paul [0000-0001-5059-4829]
dc.contributor.orcidRead, Randy [0000-0001-8273-0047]
dc.contributor.orcidRichardson, Sylvia [0000-0003-1998-492X]
dc.contributor.orcidCvejic, Ana [0000-0003-3204-9311]
dc.contributor.orcidSoranzo, Nicole [0000-0003-1095-3852]
dc.contributor.orcidOuwehand, Willem [0000-0002-7744-1790]
dc.contributor.orcidFrontini, Mattia [0000-0001-8074-6299]
dc.contributor.orcidRendon Restrepo, Augusto [0000-0001-8994-0039]
dc.identifier.eissn1095-9203
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (082961/Z/07/Z)
pubs.funder-project-idWellcome Trust (084183/Z/07/Z)
pubs.funder-project-idBritish Heart Foundation (RG/09/012/28096)
pubs.funder-project-idNIHR�Central Commissioning Facility (CCF) (RP-PG-0310-1002)
pubs.funder-project-idMRC (MR/K024043/1)
pubs.funder-project-idWellcome Trust (100140/Z/12/Z)
pubs.funder-project-idWellcome Trust (091310/Z/10/Z)
rioxxterms.freetoread.startdate2015-03-26


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