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dc.contributor.authorMagbanua, Jose Paoloen
dc.contributor.authorRunneburger, Estelleen
dc.contributor.authorRussell, Steveen
dc.contributor.authorWhite, Roberten
dc.date.accessioned2014-11-13T12:08:55Z
dc.date.available2014-11-13T12:08:55Z
dc.date.issued2014-11-03en
dc.identifier.citationMolecular and Cell Biology 2015, vol. 35 no. 1 318-330. DOI: 10.1128/MCB.01061-14en
dc.identifier.issn0270-7306
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/246374
dc.description.abstractAlthough the majority of genomic binding sites for the insulator protein CTCF are constitutively occupied, a subset show variably occupancy. Such variable sites provide an opportunity to assess context-specific CTCF functions in gene regulation. Here we have identified a variably occupied CTCF site in the Drosophila Ultrabithorax (Ubx) gene. This site is occupied in tissues where Ubx is active (third thoracic leg imaginal disc) but is not bound in tissues where the Ubx gene is repressed (first thoracic leg imaginal disc). Using chromatin conformation capture we show that this site preferentially interacts with the Ubx promoter region in the active state. The site lies close to Ubx enhancer elements and is also close to the locations of several gypsy transposon insertions that disrupt Ubx expression, leading to the bx mutant phenotype. Gypsy insertions carry the Su(Hw)-dependent gypsy insulator and were found to affect both CTCF binding at the variable site and the chromatin topology. This suggests that insertion of the gypsy insulator in this region interferes with CTCF function and supports a model for the normal function of the variable CTCF site as a chromatin loop facilitator, promoting interaction between Ubx enhancers and the Ubx transcription start site.
dc.description.sponsorshipThe work was supported by the Wellcome Trust [089834/Z/09/Z] and ER was supported by the Erasmus Programme.
dc.languageEnglishen
dc.language.isoenen
dc.publisherAmerican Society for Microbiology
dc.titleA variably occupied CTCF binding site in the Ultrabithorax gene in the Drosophila Bithorax Complexen
dc.typeArticle
dc.description.versionThis is the accepted manuscript. The final version is available from ASM at http://mcb.asm.org/content/early/2014/10/28/MCB.01061-14.abstract.en
prism.endingPage330
prism.publicationDate2014en
prism.publicationNameMolecular and Cellular Biologyen
prism.startingPage318
prism.volume35en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.projectid089834/Z/09/Z
dcterms.dateAccepted2014-10-25en
rioxxterms.versionofrecord10.1128/MCB.01061-14en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-11-03en
dc.contributor.orcidRussell, Steve [0000-0003-0546-3031]
dc.contributor.orcidWhite, Robert [0000-0002-0019-8227]
dc.identifier.eissn1098-5549
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (089834/Z/09/Z)
rioxxterms.freetoread.startdate2015-05-03


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