How are pluripotent cells captured in culture?
Reproductive Medicine and Biology
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Kinoshita, M. (2014). How are pluripotent cells captured in culture?. Reproductive Medicine and Biology, 14 85-98. https://doi.org/10.1007/s12522-014-0199-8
In mice, three pluripotent stem cell lines have been established from different stage of developing embryo, which are embryonic stem (ES) cell, post-implantation epiblast stem cell (EpiSC) and embryonic germ (EG) cell. ES cell and EG cells share many common features including factor requirement, colony morphology and gene expression pattern. On the other hand, EpiSC needs different external signal inputs, exhibits flattened colony morphology and a different set of gene expression patterns. In addition, the germ line competency of EpiSCs is still unclear. To distinguish the differences between them, they are defined by the words, “naïve” and “primed” pluripotent cells respectively. This article introduces how pluripotent stem cell lines are established in culture, and how much those cells in vitro are similar or relevant to their in vivo origin and the knowledge about transcription factors to support this state.
EG cells, Epiblast, EpiSC, ES cell, Pluripotency
The author works in the laboratory of Austin Smith, funded by the Wellcome Trust and Medical Research Council.
External DOI: https://doi.org/10.1007/s12522-014-0199-8
This record's URL: https://www.repository.cam.ac.uk/handle/1810/246420
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/