A joint Richardson—Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data
Methods and Applications in Fluorescence
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Ströhl, F., & Kaminski, C. F. (2015). A joint Richardson—Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data. Methods and Applications in Fluorescence, 3 014002. https://doi.org/10.1088/2050-6120/3/1/014002
We demonstrate the reconstruction of images obtained by multifocal structured illumination microscopy, MSIM, using a joint Richardson–Lucy, jRL-MSIM, deconvolution algorithm, which is based on an underlying widefield image-formation model. The method is efficient in the suppression of out-of-focus light and greatly improves image contrast and resolution. Furthermore, it is particularly well suited for the processing of noise corrupted data. The principle is verified on simulated as well as experimental data and a comparison of the jRL-MSIM approach with the standard reconstruction procedure, which is based on image scanning microscopy, ISM, is made. Our algorithm is efficient and freely available in a user friendly software package.
superresolution, image processing, structured illumination microscopy
This work was supported by grants from the Leverhulme Trust, the Engineering and Physical Sciences Research Council, UK (grant EP/H018301/1) and by the Medical Research Council (grant MR/K015850/1). FS wishes to acknowledge support from the Studienstiftung des deutschen Volkes and the Erlangen Graduate School in Advanced Optical Technologies (SAOT) by the German Research Foundation (DFG).
External DOI: https://doi.org/10.1088/2050-6120/3/1/014002
This record's URL: https://www.repository.cam.ac.uk/handle/1810/246792
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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