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dc.contributor.authorOefner, CMen
dc.contributor.authorSharkey, Andrewen
dc.contributor.authorGardner, Len
dc.contributor.authorCritchley, Hen
dc.contributor.authorOyen, Michelleen
dc.contributor.authorMoffett, Ashleyen
dc.date.accessioned2015-03-19T10:20:10Z
dc.date.available2015-03-19T10:20:10Z
dc.date.issued2014-11-01en
dc.identifier.citationPlacenta Volume 36, Issue 1, January 2015, Pages 59–68. DOI: 10.1016/j.placenta.2014.10.012en
dc.identifier.issn0143-4004
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/247075
dc.description.abstractIntroduction Extracellular matrix proteins play a crucial role in influencing the invasion of trophoblast cells. However the role of collagens and collagen type IV (col-IV) in particular at the implantation site is not clear. Methods Immunohistochemistry was used to determine the distribution of collagen types I, III, IV and VI in endometrium and decidua during the menstrual cycle and the first trimester of pregnancy. Expression of col-IV alpha chains during the reproductive cycle was determined by qPCR and protein localisation by immunohistochemistry. The structure of col-IV in placenta was examined using transmission electron microscopy. Finally, the expression of col-IV alpha chain NC1 domains and collagen receptors was localised by immunohistochemistry. Results Col-IV alpha chains were selectively up-regulated during the menstrual cycle and decidualisation. Primary extravillous trophoblast cells express collagen receptors and secrete col-IV in vitro and in vivo, resulting in the increased levels found in decidua basalis compared to decidua parietalis. A novel expression pattern of col-IV in the mesenchyme of placental villi, as a three-dimensional network, was found. NC1 domains of col-IV alpha chains are known to regulate tumour cell migration and the selective expression of these domains in decidua basalis compared to decidua parietalis was determined. Discussion Col-IV is expressed as novel forms in the placenta. These findings suggest that col-IV not only represents a structural protein providing tissue integrity but also influences the invasive behaviour of trophoblast cells at the implantation site.
dc.description.sponsorshipThis work was supported by funding from the Wellcome Trust [090108/Z/09/Z], [085992/Z/08/Z] and the British Heart Foundation [PG/09/077/27964]. C.M. Oefner was in receipt of a German National Academic Foundation PhD studentship. The authors also thank the Centre for Trophoblast Research for generous support.
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/uk/*
dc.subjectAlpha(IV) NC1 domainsen
dc.subjectExtracellular matrixen
dc.subjectPlacental beden
dc.subjectReproductive cycleen
dc.subjectTrophoblasten
dc.titleCollagen type IV at the fetal-maternal interfaceen
dc.typeArticle
dc.description.versionThis is the final published version of the article. It first appeared at http://www.sciencedirect.com/science/article/pii/S0143400414008248#.en
prism.endingPage68
prism.publicationDate2014en
prism.publicationNamePlacentaen
prism.startingPage59
prism.volume36en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderBHF
dc.rioxxterms.projectid090108/Z/09/Z
dc.rioxxterms.projectid085992/Z/08/Z
dc.rioxxterms.projectidPG/09/077/27964
dcterms.dateAccepted2014-10-21en
rioxxterms.versionofrecord10.1016/j.placenta.2014.10.012en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-11-01en
dc.contributor.orcidSharkey, Andrew [0000-0002-5072-7748]
dc.contributor.orcidOyen, Michelle [0000-0002-3428-748X]
dc.contributor.orcidMoffett, Ashley [0000-0002-8388-9073]
dc.identifier.eissn1532-3102
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (085992/Z/08/Z)
pubs.funder-project-idWellcome Trust (090108/Z/09/Z)
pubs.funder-project-idBritish Heart Foundation (None)


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