Implication of sperm RNAs in transgenerational inheritance of the effects of early trauma in mice.
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Article
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Abstract
Small non-coding RNAs (sncRNAs) are potential vectors at the interface between genes and environment. We found that traumatic stress in early life altered mouse microRNA (miRNA) expression, and behavioral and metabolic responses in the progeny. Injection of sperm RNAs from traumatized males into fertilized wild-type oocytes reproduced the behavioral and metabolic alterations in the resulting offspring.
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Keywords
Animals, Animals, Newborn, Cell Line, Tumor, Dark Adaptation, Disease Models, Animal, Energy Intake, Female, Gene Expression Regulation, Male, Maternal Deprivation, Maze Learning, Mice, Mice, Inbred C57BL, MicroRNAs, Oocytes, RNA, Small Interfering, RNA, Small Untranslated, Reaction Time, Spermatozoa, Stress, Psychological
Journal Title
Nat Neurosci
Conference Name
Journal ISSN
1097-6256
1546-1726
1546-1726
Volume Title
17
Publisher
Springer Science and Business Media LLC
Publisher DOI
Sponsorship
Wellcome Trust (092096/Z/10/Z)
We thank M. Rassoulzadegan and V. Grandjean for help with the sperm
purification, F. Manuella and H. Hörster for assistance with the MSUS paradigm,
H. Welzl for help with behavior, G. Vernaz for help with western blotting,
R. Tweedie-Cullen and P. Nanni for help with mass spectrometry, A. Patrignani
for advice on DNA and RNA quality assessment, and A. Chen and A. Brunner for
constructive discussions. This work was supported by the Austrian Academy of
Sciences, the University of Zürich, the Swiss Federal Institute of Technology,
Roche, the Swiss National Science Foundation, and The National Center of
Competence in Research “Neural Plasticity and Repair”. P.S. was supported by a
Gonville and Caius College fellowship.