Formation and Abundance of 5-Hydroxymethylcytosine in RNA
Huber, Sabrina M
van, Delft Pieter
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Huber, S. M., van, D. P., Mendil, L., Bachman, M., Smollett, K., Werner, F., Miska, E., & et al. (2015). Formation and Abundance of 5-Hydroxymethylcytosine in RNA. ChemBioChem, 16 752-755. https://doi.org/10.1002/cbic.201500013
RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Furthermore, we have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells.
5-hydroxymethylcytosine, 5-methylcytosine, isotope tracing, LC-MS/MS, RNA modifications
This work was supported by the Cambridge PhD Training Programme in Chemical Biology and Molecular Medicine and the Wellcome Trust (grant number 099232/Z/12/Z). Dr. Donna Bond is thanked for the provision of A. thaliana total RNA and Drs. Santiago Uribe-Lewis and Adele Murrell are acknowledged for the labelled mouse tissue supply.
Wellcome Trust (099232/Z/12/Z)
External DOI: https://doi.org/10.1002/cbic.201500013
This record's URL: https://www.repository.cam.ac.uk/handle/1810/247617
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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