Formation and abundance of 5-hydroxymethylcytosine in RNA.
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Authors
Huber, Sabrina M
van Delft, Pieter https://orcid.org/0000-0002-4726-9467
Mendil, Lee
Bachman, Martin
Smollett, Katherine
Abstract
RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcytidine (f(5)C). Furthermore, we have identified hm(5)C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m(5)C oxidation is a conserved process that could have critical regulatory functions inside cells.
Description
Keywords
5-hydroxymethylcytosine, 5-methylcytosine, LC-MS/MS, RNA modifications, isotope tracing, 5-Methylcytosine, Animals, Chromatography, High Pressure Liquid, Cytosine, Mice, Mice, Inbred C57BL, Molecular Structure, Oxidation-Reduction, RNA, Tandem Mass Spectrometry
Journal Title
Chembiochem
Conference Name
Journal ISSN
1439-4227
1439-7633
1439-7633
Volume Title
16
Publisher
Wiley
Publisher DOI
Sponsorship
Wellcome Trust (099232/Z/12/Z)
Wellcome Trust (104640/Z/14/Z)
Wellcome Trust (104640/Z/14/Z)
This work was supported by the Cambridge PhD Training Programme
in Chemical Biology and Molecular Medicine and the
Wellcome Trust (grant number 099232/Z/12/Z). Dr. Donna Bond
is thanked for the provision of A. thaliana total RNA and Drs.
Santiago Uribe-Lewis and Adele Murrell are acknowledged for
the labelled mouse tissue supply.