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dc.contributor.authorHuber, Sabrina Men
dc.contributor.authorvan, Delft Pieteren
dc.contributor.authorMendil, Leeen
dc.contributor.authorBachman, Martinen
dc.contributor.authorSmollett, Katherineen
dc.contributor.authorWerner, Finnen
dc.contributor.authorMiska, Ericen
dc.contributor.authorBalasubramanian, Shankaren
dc.date.accessioned2015-05-12T11:31:55Z
dc.date.available2015-05-12T11:31:55Z
dc.date.issued2015-02-12en
dc.identifier.citationChemBioChem, 16: 752–755. doi: 10.1002/cbic.201500013en
dc.identifier.issn1439-4227
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/247617
dc.description.abstractRNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Furthermore, we have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells.
dc.description.sponsorshipThis work was supported by the Cambridge PhD Training Programme in Chemical Biology and Molecular Medicine and the Wellcome Trust (grant number 099232/Z/12/Z). Dr. Donna Bond is thanked for the provision of A. thaliana total RNA and Drs. Santiago Uribe-Lewis and Adele Murrell are acknowledged for the labelled mouse tissue supply.
dc.languageEnglishen
dc.language.isoenen
dc.publisherWiley
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.subject5-hydroxymethylcytosineen
dc.subject5-methylcytosineen
dc.subjectisotope tracingen
dc.subjectLC-MS/MSen
dc.subjectRNA modificationsen
dc.titleFormation and Abundance of 5-Hydroxymethylcytosine in RNAen
dc.typeArticle
dc.description.versionThis is the final published version. It first appeared at http://onlinelibrary.wiley.com/doi/10.1002/cbic.201500013/abstract;jsessionid=61B9B3D8937FE50CFA4954A4C4B445B6.f02t04.en
prism.endingPage755
prism.publicationDate2015en
prism.publicationNameChemBioChemen
prism.startingPage752
prism.volume16en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.projectid099232/Z/12/Z
rioxxterms.versionofrecord10.1002/cbic.201500013en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-02-12en
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn1439-7633
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (099232/Z/12/Z)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales