Validation of an automated enzyme immunoassay for the measurement of serum total thyroxine in cats
Veterinary Clinical Pathology
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Williams, T., & Archer, J. (2016). Validation of an automated enzyme immunoassay for the measurement of serum total thyroxine in cats. Veterinary Clinical Pathology, 45 148-153. https://doi.org/10.1111/vcp.12324
Background: Hyperthyroidism is common in older cats, which necessitates frequent screening of serum total thyroxine (TT4) concentrations. Fast, cheap, and reliable ways to measure TT4 in cats are needed. Objectives: Validation of a human TT4 enzyme immunoassay (EIA) for use with feline serum and derivation of a TT4 reference interval (RI) for cats aged 9 years and older). Methods: Assay precision, reproducibility, and linearity were evaluated. Interference by hemolysis was also assessed. Method comparison studies between the EIA and previously validated radioimmunoassay (RIA) and chemiluminescent-enzyme immunoassay (CEIA) were performed. Healthy cats (>9 years) were recruited from three UK first opinion practices. Results: The human TT4 EIA demonstrated good precision and reproducibility and adequate linearity. Hemolysis did not significantly alter measured TT4 concentrations until hemoglobin concentration exceeded 8 g/L. Method comparison revealed proportional and constant error between EIA and RIA/CEIA. The TT4 RI for cats (>9 years) was calculated as 7.1-45.1 nmol/L (n=49). Conclusions: The human TT4 EIA was successfully validated for use with feline serum and offers a rapid, cheap and reliable method for determination of serum TT4 concentrations in cats.
hyperthyroidism, feline, reference interval
External DOI: https://doi.org/10.1111/vcp.12324
This record's URL: https://www.repository.cam.ac.uk/handle/1810/247747