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dc.contributor.authorAkay, Alper
dc.contributor.authorSarkies, Peter
dc.contributor.authorMiska, Eric
dc.date.accessioned2015-05-20T09:11:20Z
dc.date.available2015-05-20T09:11:20Z
dc.date.issued2015-04-15
dc.identifier.citationScientific Reports 5, 2015, Article number: 9597. DOI: 10.1038/srep09597
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/247834
dc.description.abstractThe discovery of RNA interference (RNAi) in C. elegans has had a major impact on scientific research, led to the rapid development of RNAi tools and has inspired RNA-based therapeutics. Astonishingly, nematodes, planaria and many insects take up double-stranded RNA (dsRNA) from their environment to elicit RNAi; the biological function of this mechanism is unclear. Recently, the E. coli OxyS non-coding RNA was shown to regulate gene expression in C. elegans when E. coli is offered as food. This was surprising given that C. elegans is unlikely to encounter E. coli in nature. To directly test the hypothesis that the E. coli OxyS non-coding RNA triggers the C. elegans RNAi pathway, we sequenced small RNAs from C. elegans after feeding with bacteria. We clearly demonstrate that the OxyS non-coding RNA does not trigger an RNAi response in C. elegans. We conclude that the biology of environmental RNAi remains to be discovered.
dc.description.sponsorshipPS is funded by a research fellowship from the Gonville and Caius College, University of Cambridge. AA and EAM are supported by a Cancer Research UK programme grant to EAM.
dc.languageEnglish
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleE. coli OxyS non-coding RNA does not trigger RNAi in C. elegans.
dc.typeArticle
dc.description.versionThis is the final published version. It first appeared at http://www.nature.com/srep/2015/150411/srep09597/full/srep09597.html.
prism.number9597
prism.publicationDate2015
prism.publicationNameSci Rep
prism.volume5
dc.rioxxterms.funderCRUK
dcterms.dateAccepted2015-03-09
rioxxterms.versionofrecord10.1038/srep09597
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-04-15
dc.contributor.orcidAkay, Alper [0000-0001-6825-4443]
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
cam.issuedOnline2015-04-15


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales