Targeted Detection of G-Quadruplexes in Cellular RNAs
Kwok, Chun Kit
Angewandte Chemie International Edition
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Kwok, C. K., & Balasubramanian, S. (2015). Targeted Detection of G-Quadruplexes in Cellular RNAs. Angewandte Chemie International Edition, 54 6751-6754. https://doi.org/10.1002/anie.201500891
The G-quadruplex (G4) is a non-canonical nucleic acid structure which regulates important cellular processes. RNA G4s have recently been shown to exist in human cells and be biologically significant. Described herein is a new approach to detect and map RNA G4s in cellular transcripts. This method exploits the specific control of RNA G4–cation and RNA G4–ligand interactions during reverse transcription, by using a selective reverse transcriptase to monitor RNA G4-mediated reverse transcriptase stalling (RTS) events. Importantly, a ligation-amplification strategy is coupled with RTS, and enables detection and mapping of G4s in important, low-abundance cellular RNAs. Strong evidence is provided for G4 formation in full-length cellular human telomerase RNA, offering important insights into its cellular function.
cations, G-quadruplexes, ligand effects, reverse transcription, RNA structures, telomerase RNA
This study is supported by a European Research Council Advanced grant to S.B. and supports C.K.K., and the Croucher Foundation for a fellowship to C.K.K. We thank Dr. M. Di Antonio, V. Chambers, and G. Mclnroy for providing comments on the manuscript.
External DOI: https://doi.org/10.1002/anie.201500891
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248019
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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