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dc.contributor.authorGornik, Sebastian G
dc.contributor.authorFebrimarsa
dc.contributor.authorCassin, Andrew M
dc.contributor.authorMacRae, James I
dc.contributor.authorRamaprasad, Abhinay
dc.contributor.authorRchiad, Zineb
dc.contributor.authorMcConville, Malcolm J
dc.contributor.authorBacic, Antony
dc.contributor.authorMcFadden, Geoffrey I
dc.contributor.authorPain, Arnab
dc.contributor.authorWaller, Ross
dc.date.accessioned2015-05-29T11:37:47Z
dc.date.available2015-05-29T11:37:47Z
dc.date.issued2015-05-05
dc.identifier.citationGornik et al., PNAS 2015, 112, 18, 5767-5772, doi:10.1073/pnas.1423400112
dc.identifier.issn0027-8424
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248088
dc.description.abstractOrganelle gain through endosymbiosis has been integral to the origin and diversification of eukaryotes, and, once gained, plastids and mitochondria seem seldom lost. Indeed, discovery of nonphotosynthetic plastids in many eukaryotes--notably, the apicoplast in apicomplexan parasites such as the malaria pathogen Plasmodium--highlights the essential metabolic functions performed by plastids beyond photosynthesis. Once a cell becomes reliant on these ancillary functions, organelle dependence is apparently difficult to overcome. Previous examples of endosymbiotic organelle loss (either mitochondria or plastids), which have been invoked to explain the origin of eukaryotic diversity, have subsequently been recognized as organelle reduction to cryptic forms, such as mitosomes and apicoplasts. Integration of these ancient symbionts with their hosts has been too well developed to reverse. Here, we provide evidence that the dinoflagellate Hematodinium sp., a marine parasite of crustaceans, represents a rare case of endosymbiotic organelle loss by the elimination of the plastid. Extensive RNA and genomic sequencing data provide no evidence for a plastid organelle, but, rather, reveal a metabolic decoupling from known plastid functions that typically impede organelle loss. This independence has been achieved through retention of ancestral anabolic pathways, enzyme relocation from the plastid to the cytosol, and metabolic scavenging from the parasite's host. Hematodinium sp. thus represents a further dimension of endosymbiosis--life after the organelle.
dc.description.sponsorshipWe thank Nick Katris for assistance with Toxoplasma transformation and Ellen Nisbet for critically reading this report. This work was supported by Australian Research Council (ARC) Grants DP130100572 and DP1093395; a King Abdullah University of Science and Technology Faculty Baseline Research Fund; and Victorian Life Sciences Computation Initiative Grant VR0254. S.G.G. was supported by Science Foundation Ireland Grant 13/SIRG/2125; F. was supported by an Australia Award; A.M.C. and A.B. were supported by ARC Centre of Excellence in Plant Cell Walls Grant CE110001007; and M.J.M. was supported by the National Health and Medical Research Council as a Principal Research Fellow.
dc.languageEnglish
dc.language.isoen
dc.publisherProceedings of the National Academy of Sciences
dc.subjectorganelle loss
dc.subjectplastid loss
dc.subjectendosymbiosis
dc.subjectplastid metabolism
dc.subjectdiaminopimelate aminotransferase
dc.titleEndosymbiosis undone by stepwise elimination of the plastid in a parasitic dinoflagellate.
dc.typeArticle
dc.description.versionThis is the accepted manuscript of a paper published in the Proceedings of the National Academy of Sciences (Gornik et al., PNAS 2015, 112, 18, 5767-5772, doi:10.1073/pnas.1423400112). The final version is available at http://dx.doi.org/10.1073/pnas.1423400112
prism.endingPage5772
prism.number18
prism.publicationDate2015
prism.publicationNameProc Natl Acad Sci U S A
prism.startingPage5767
prism.volume112
dc.rioxxterms.funderARC
dc.rioxxterms.funderKing Abdullah University of Science and Technology Faculty Baseline Research Fund
dc.rioxxterms.funderVictorian Life Sciences Computation Initiative
dc.rioxxterms.funderScience Foundation Ireland
dc.rioxxterms.funderAustralia Award
dc.rioxxterms.funderNHMRC
dc.rioxxterms.projectidDP130100572
dc.rioxxterms.projectidDP1093395
dc.rioxxterms.projectidVR0254
dc.rioxxterms.projectid13/SIRG/2125
dc.rioxxterms.projectidCE110001007
rioxxterms.versionofrecord10.1073/pnas.1423400112
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-04-20
dc.contributor.orcidWaller, Ross [0000-0001-6961-9344]
dc.identifier.eissn1091-6490
rioxxterms.typeJournal Article/Review
cam.issuedOnline2015-04-20
rioxxterms.freetoread.startdate2015-10-20


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