The Response of Greek Key Proteins to Changes in Connectivity Depends on the Nature of Their Secondary Structure
View / Open Files
Publication Date
2015-04-07Journal Title
Journal of Molecular Biology
ISSN
0022-2836
Publisher
Elsevier
Volume
427
Pages
2159-2165
Language
English
Type
Article
Metadata
Show full item recordCitation
Kemplen, K., De, S. D., & Clarke, J. (2015). The Response of Greek Key Proteins to Changes in Connectivity Depends on the Nature of Their Secondary Structure. Journal of Molecular Biology, 427 2159-2165. https://doi.org/10.1016/j.jmb.2015.03.020
Abstract
What governs the balance between connectivity and topology in regulating the mechanism of protein folding? We use circular permutation to vary the order of the helices in the all-α Greek key protein FADD (Fas-associated death domain) to investigate this question. Unlike all-β Greek key proteins, where changes in the order of secondary structure cause a shift in the folding nucleus, the position of the nucleus in FADD is unchanged, even when permutation reduces the complexity significantly. We suggest that this is because local helical contacts are so dominant that permutation has little effect on the entropic cost of forming the folding nucleus whereas, in all-β Greek key proteins, all interactions in the nucleus are long range. Thus, the type of secondary structure modulates the sensitivity of proteins to changes in connectivity.
Keywords
protein folding, topology, death domain, immunoglobulin fold, phi-value
Sponsorship
This work was supported by the Wellcome Trust (WT095195) (J.C), Engineering and Physical Sciences Research Council (UK) Grant EP/J016764/1 (D.D.S.) and an Engineering and Physical Sciences Research Council (UK) studentship (K.R.K.). J.C. is a Wellcome Trust Senior Research fellow.
Funder references
EPSRC (EP/J016764/1)
Wellcome Trust (095195/Z/10/Z)
Identifiers
External DOI: https://doi.org/10.1016/j.jmb.2015.03.020
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248198
Rights
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
Recommended or similar items
The following licence files are associated with this item: