Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance.
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Publication Date
2015-04-15Journal Title
Journal of the Royal Soiety. Interface
ISSN
1742-5689
Publisher
Royal Society Publishing
Volume
12
Number
20141389
Language
English
Type
Article
Metadata
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Prasad, A., Huefner, A., Mahajan, S., & Seshia, A. (2015). Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance.. Journal of the Royal Soiety. Interface, 12 (20141389)https://doi.org/10.1098/rsif.2014.1389
Abstract
Quantifying cellular behaviour by motility and morphology changes is increasingly important in formulating an understanding of fundamental physiological phenomena and cellular mechanisms of disease. However, cells are complex biological units, which often respond to external environmental factors by manifesting subtle responses that may be difficult to interpret using conventional biophysical measurements. This paper describes the adaptation of the quartz crystal microbalance (QCM) to monitor neuroblastoma cells undergoing environmental stress wherein the frequency stability of the device can be correlated to changes in cellular state. By employing time domain analysis of the resulting frequency fluctuations, it is possible to study the variations in cellular motility and distinguish between different cell states induced by applied external heat stress. The changes in the frequency fluctuation data are correlated to phenotypical physical response recorded using optical microscopy under identical conditions of environmental stress. This technique, by probing the associated biomechanical noise, paves the way for its use in monitoring cell activity, and intrinsic motility and morphology changes, as well as the modulation resulting from the action of drugs, toxins and environmental stress.
Identifiers
External DOI: https://doi.org/10.1098/rsif.2014.1389
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248228
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