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Synthetic long peptide booster immunization in rhesus macaques primed with replication-competent NYVAC-C-KC induces a balanced CD4/CD8 T-cell and antibody response against the conserved regions of HIV-1.


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Authors

Mooij, Petra 
Koopman, Gerrit 
Drijfhout, Jan Wouter 
Nieuwenhuis, Ivonne G 
Beenhakker, Niels 

Abstract

The Thai trial (RV144) indicates that a prime-boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a pox-vector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFNγ T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFNγ ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.

Description

Keywords

AIDS Vaccines, Animals, Antibody Formation, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, HIV Antibodies, HIV-1, Immunization, Secondary, Macaca mulatta, Vaccines, Synthetic

Journal Title

J Gen Virol

Conference Name

Journal ISSN

0022-1317
1465-2099

Volume Title

96

Publisher

Microbiology Society
Sponsorship
This project was conducted under the auspices of of the Poxvirus T-cell Vaccine Discovery Consortium (PTVDC) as part of the Collaboration for AIDS Vaccine Discovery (CAVD) with support from the Bill and Melinda Gates Foundation.