XL-MS: Protein cross-linking coupled with mass spectrometry
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Holding, A. (2015). XL-MS: Protein cross-linking coupled with mass spectrometry. Methods, 89 54-63. https://doi.org/10.1016/j.ymeth.2015.06.010
With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions.
Proteomics, Mass spectrometry, Structure, Quantitation, Systems biology, Cross-linking, XL-MS
This work was support by the Medical Research Council – United Kingdom.
External DOI: https://doi.org/10.1016/j.ymeth.2015.06.010
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248661
Creative Commons Attribution 4.0
Licence URL: http://creativecommons.org/licenses/by/4.0/
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