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dc.contributor.authorLi, Yangen
dc.contributor.authorReid, Daviden
dc.contributor.authorBazin, Dominiqueen
dc.contributor.authorDoudon, Michelen
dc.contributor.authorDuer, Melindaen
dc.date.accessioned2015-07-13T14:15:33Z
dc.date.available2015-07-13T14:15:33Z
dc.date.issued2015-07-13en
dc.identifier.issn1631-0748
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248906
dc.description.abstractSolid state NMR (ssNMR) can characterize mineral (31P) and organic (13C) components of human salivary stones (n = 8). All show apatitic 31P spectra. 13C ssNMR indicates more protein, of more consistent composition, than apatitic uroliths, with signals from Tyr, Phe and His prominent. Citrate and lipid, identified by dipolar dephasing (DD), and polysaccharides are also observable in varying amounts. 13C{31P} rotational echo double resonance (13C{31P} REDOR) identifies carbon atoms in close (< ca. 0.5 nm) proximity to phosphorus and therefore probably binding with mineral. Citrate, sugar and carboxylate signals undergo strong 13C{31P} REDOR, also seen to signals between 50 and 60 ppm, from protein α- carbons and, possibly, phosphoserines and phospholipids, and sometimes to a 35 – 40 ppm envelope containing Asp-Cβ and Glu-Cγ signals. Amino acid analysis indicates high proline and aromatic content. 13C ssNMR and amino acid composition is consistent with preponderance of proline-rich salivary proteins such as statherin.
dc.description.sponsorshipThe U.K. EPSRC (Y. L.) and MRC (D. G. R.) for funding
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/uk/*
dc.subjectApatiteen
dc.subjectCitrateen
dc.subjectPolysaccharidesen
dc.subjectProline-rich proteinsen
dc.subjectREDORen
dc.subjectSalivary proteinsen
dc.subjectStatherinen
dc.titleSolid state NMR of salivary calculi: proline-rich salivary proteins, citrate, polysaccharide, lipid, and organic-mineral interactionsen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.crci.2015.07.001en
prism.publicationDate2015en
prism.publicationNameComptes Rendus Chimieen
dc.rioxxterms.funderEPSRC
dc.rioxxterms.funderMRC
dcterms.dateAccepted2015-07-03en
rioxxterms.versionofrecord10.1016/j.crci.2015.07.001en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-07-13en
dc.contributor.orcidReid, David [0000-0003-2464-5295]
dc.contributor.orcidDuer, Melinda [0000-0001-6594-8917]
dc.identifier.eissn1878-1543
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2017-01-03


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