Preparation and supramolecular recognition of multivalent peptide-polysaccharide conjugates by cucurbituril in hydrogel formation
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Rowland, M. J., Atgie, M., Hoogland, D., & Scherman, O. (2015). Preparation and supramolecular recognition of multivalent peptide-polysaccharide conjugates by cucurbituril in hydrogel formation. Biomacromolecules, 16 2436-2443. https://doi.org/10.1021/acs.biomac.5b00680
Supramolecular hydrogels were fabricated by physically cross-linking phenylalanine functionalized polysaccharides with cucurbituril in water. We report a facile 2-step method of functionalization of the polysaccharides hyaluronic acid (HA), carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and guar with the dipeptide Phe-Cys. Addition of cucurbituril to the functional polysaccharides initiated physical cross-linking on account of strong 1:2 "hetero-ternary" complexes with the pendant Phe residues. In particular, HA and CMC based soft hydrogels displayed impressive viscoelastic behavior which was characterized using rheology, demonstrating accessibility to an array of material properties which would find broad applicability to many fields.
M.J.R. thanks the University of Cambridge Chemical Biology and Molecular Medicine PhD Training Programme for funding. OAS thanks ERC Starting Investigator Grant (ASPiRe). The authors would also like to thank Silvia Sonzini for her assistance with collecting ITC data.
European Research Council (240629)
External DOI: https://doi.org/10.1021/acs.biomac.5b00680
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248956