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dc.contributor.authorColin, Pierre-Yvesen
dc.contributor.authorZinchenko, Anastasiaen
dc.contributor.authorHollfelder, Florianen
dc.date.accessioned2015-07-23T15:50:14Z
dc.date.available2015-07-23T15:50:14Z
dc.date.issued2015-09-03en
dc.identifier.citationCurrent Opinion in Structural Biology 2015, 33, 42-51. doi: 10.1016/j.sbi.2015.06.001
dc.identifier.issn0959-440X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/249044
dc.description.abstractThe success of a directed evolution approach to creating custom-made enzymes relies in no small part on screening as many clones as possible. The miniaturisation of assays into pico- to femtoliter compartments (emulsion droplets, vesicles or gel-shell beads) makes directed evolution campaigns practically more straightforward than current large scale industrial screening that requires liquid handling equipment and much manpower. Several recent experimental formats have established protocols to screen more than 10 million compartments per day, representing unprecedented throughput at low cost. This review introduces the emerging approaches towards making biomimetic man-made compartments that are poised to be adapted by a wider circle of researchers. In addition to cost and time saving, control of selection pressures and conditions, the quantitative readout that reports on every library members and the ability to develop strategies based on these data will increase the degrees of freedom in designing and testing strategies for directed evolution experiments.
dc.description.sponsorshipOur research was funded by the Biological and Biotechnological Research Council (BBSRC) and the Engineering and Physical Sciences Research Council (EPSRC). FH is an ERC Starting Investigator, PYC and AZ were supported by the EU Marie-Curie training network PhosChemRec. AZ received further support from the BBSRC and the Cambridge European Trust.
dc.languageEnglishen
dc.language.isoenen
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/uk/*
dc.titleEnzyme Engineering in Biomimetic Compartmentsen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.sbi.2015.06.001en
prism.endingPage51
prism.publicationDate2015en
prism.publicationNameCurrent Opinion in Structural Biologyen
prism.startingPage42
prism.volume33en
dc.rioxxterms.funderEPSRC
dc.rioxxterms.funderBBSRC
dc.rioxxterms.funderERC
dc.rioxxterms.funderCambridge European Trust
dcterms.dateAccepted2015-06-04en
rioxxterms.versionofrecord10.1016/j.sbi.2015.06.001en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-09-03en
dc.contributor.orcidHollfelder, Florian [0000-0002-1367-6312]
dc.identifier.eissn1879-033X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/L002469/1)
pubs.funder-project-idEPSRC (EP/H046593/1)
rioxxterms.freetoread.startdate2016-09-03


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