The DREAM complex promotes gene body H2A.Z for target repression

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Authors
Latorre, Isabel
Chesney, Michael A
Garrigues, Jacob M
  Stempor, Przemyslaw
Appert, Alex
Francescon, Mirko
Strome, Susan
Publication Date
2015-07-01
Journal Title
Genes & Development
ISSN
0890-9369
Publisher
CSH Press
Volume
29
Pages
495-500
Language
English
Type
Article
Metadata
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Citation
Latorre, I., Chesney, M. A., Garrigues, J. M., Stempor, P., Appert, A., Francescon, M., Strome, S., & et al. (2015). The DREAM complex promotes gene body H2A.Z for target repression. Genes & Development, 29 495-500. https://doi.org/10.1101/gad.255810.114
Abstract
The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual pattern of high gene body HTZ-1/H2A.Z. In mutants of lin-35, the sole p130/Rb-like gene in C. elegans, DREAM targets have reduced gene body HTZ-1/H2A.Z and increased expression. Consistent with a repressive role for gene body H2A.Z, many DREAM targets are up-regulated in htz-1/H2A.Z mutants. Our results indicate that the DREAM complex facilitates high gene body HTZ-1/H2A.Z, which plays a role in target gene repression.
Keywords
C. elegans, H2A.Z, Retinoblastoma/DREAM, transcriptional repression
Sponsorship
We are grateful to D. Fay for providing the 5× outcrossed lin-35 strain, and Robert Horvitz for antibodies. I.L., M.A.C., P.S., A.A., and J.A. were supported by Wellcome Trust Senior Research Fellowships to J.A. (054523 and 101863). J.A. also acknowledges support by core funding from the Wellcome Trust and Cancer Research UK. J.M.G. and S.S. were supported by National Institutes of Health (NIH) R01 grant GM34059. Part of this work was supported by NIH National Human Genome Research Institute (NHGRI) grant U01 HG004270 to the modENCODE consortium headed by J.D. Lieb.
Funder references
Wellcome Trust (084598/Z/07/Z)
Wellcome Trust (101863/Z/13/Z)
Wellcome Trust (054523/Z/98/C)
Identifiers
External DOI: https://doi.org/10.1101/gad.255810.114
This record's URL: https://www.repository.cam.ac.uk/handle/1810/249060

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Creative Commons Attribution 4.0 International License
Licence URL: http://creativecommons.org/licenses/by/4.0/


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Creative Commons Attribution 4.0 International License
Except where otherwise noted, this item's licence is described as Creative Commons Attribution 4.0 International License