The DREAM complex promotes gene body H2A.Z for target repression.
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Authors
Latorre, Isabel
Chesney, Michael A
Garrigues, Jacob M
Stempor, Przemyslaw https://orcid.org/0000-0002-9464-7475
Appert, Alex
Abstract
The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual pattern of high gene body HTZ-1/H2A.Z. In mutants of lin-35, the sole p130/Rb-like gene in C. elegans, DREAM targets have reduced gene body HTZ-1/H2A.Z and increased expression. Consistent with a repressive role for gene body H2A.Z, many DREAM targets are up-regulated in htz-1/H2A.Z mutants. Our results indicate that the DREAM complex facilitates high gene body HTZ-1/H2A.Z, which plays a role in target gene repression.
Description
Keywords
C. elegans, H2A.Z, Retinoblastoma/DREAM, transcriptional repression, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Regulation, Developmental, Genes, cdc, Histones, Mutation, Protein Binding, Transcriptome
Journal Title
Genes Dev
Conference Name
Journal ISSN
0890-9369
1549-5477
1549-5477
Volume Title
29
Publisher
Cold Spring Harbor Laboratory
Publisher DOI
Sponsorship
Wellcome Trust (084598/Z/07/Z)
Wellcome Trust (101863/Z/13/Z)
Wellcome Trust (054523/Z/98/C)
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (101863/Z/13/Z)
Wellcome Trust (054523/Z/98/C)
Wellcome Trust (092096/Z/10/Z)
We are grateful to D. Fay for providing the 5× outcrossed lin-35 strain, and Robert Horvitz for antibodies. I.L., M.A.C., P.S., A.A., and J.A. were supported by Wellcome Trust Senior Research Fellowships to J.A. (054523 and 101863). J.A. also acknowledges support by core funding from the Wellcome Trust and Cancer Research UK. J.M.G. and S.S. were supported by National Institutes of Health (NIH) R01 grant GM34059. Part of this work was supported by NIH National Human Genome Research Institute (NHGRI) grant U01 HG004270 to the modENCODE consortium headed by J.D. Lieb.