Epigenetic analysis of regulatory T cells using multiplex bisulfite sequencing.
Change log
Authors
Rainbow, Daniel B
Yang, Xin
Pekalski, Marcin L
Smyth, Deborah J
Description
Keywords
Bisulfite sequencing, FOXP3, Methylation, Next-generation sequencing, Regulatory T cell, DNA Methylation, Epigenesis, Genetic, Female, Forkhead Transcription Factors, High-Throughput Nucleotide Sequencing, Humans, Male, Polymerase Chain Reaction, Sulfites, T-Lymphocytes, Regulatory
Journal Title
Eur J Immunol
Conference Name
Journal ISSN
0014-2980
1521-4141
1521-4141
Volume Title
45
Publisher
Wiley
Publisher DOI
Sponsorship
Wellcome Trust (089989/Z/09/Z)
Wellcome Trust (100140/Z/12/Z)
Wellcome Trust (091157/Z/10/B)
Wellcome Trust (100140/Z/12/Z)
Wellcome Trust (091157/Z/10/B)
This work was supported by Wellcome Trust Grant 096388, JDRF Grant 9-2011-253, the National Institute for Health Research Cambridge Biomedical Research Centre (BRC) and Award P01AI039671 (to LSW. and JAT.) from the National Institute of Allergy and Infectious Diseases (NIAID). CW is supported by the Wellcome Trust (089989). The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of NIAID or the National Institutes of Health. The Cambridge Institute for Medical Research is in receipt of Wellcome Trust Strategic Award 100140. We gratefully acknowledge the participation of all NIHR Cambridge BioResource volunteers. We thank the Cambridge BioResource staff for their help with volunteer recruitment. We thank members of the Cambridge BioResource SAB and Management Committee for their support of our study and the National Institute for Health Research Cambridge Biomedical Research Centre for funding. We thank Fay Rodger and Ruth Littleboy for running the Illumina MiSeq in the Molecular Genetics Laboratories, Addenbrooke's Hospital, Cambridge. This research was supported by the Cambridge NIHR BRC Cell Phenotyping Hub. In particular, we wish to thank Anna Petrunkina Harrison, Simon McCallum, Christopher Bowman, Natalia Savinykh, Esther Perez and Jelena Markovic Djuric for their advice and support in cell sorting. We also thank Helen Stevens, Pamela Clarke, Gillian Coleman, Sarah Dawson, Jennifer Denesha, Simon Duley, Meeta Maisuria-Armer and Trupti Mistry for acquisition and preparation of samples.