When two is not enough: a CtIP tetramer is required for DNA repair by Homologous Recombination
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Publication Date
2015-08-25Journal Title
Nucleus
ISSN
1949-1034
Publisher
Taylor & Francis
Volume
6
Pages
344-348
Language
English
Type
Article
Metadata
Show full item recordCitation
Forment, J., Jackson, S., & Pellegrini, L. (2015). When two is not enough: a CtIP tetramer is required for DNA repair by Homologous Recombination. Nucleus, 6 344-348. https://doi.org/10.1080/19491034.2015.1086050
Abstract
Homologous recombination (HR) is central to the repair of double-strand DNA breaks that occur in S/G2 phases of the cell cycle. HR relies on the CtIP protein (Ctp1 in fission yeast, Sae2 in budding yeast) for resection of DNA ends, a key step in generating the 3'-DNA overhangs that are required for the HR strand-exchange reaction. Although much has been learned about the biological importance of CtIP in DNA repair, our mechanistic insight into its molecular functions remains incomplete. It has been recently discovered that CtIP and Ctp1 share a conserved tetrameric architecture that is mediated by their N-terminal domains and is critical for their function in HR. The specific arrangement of protein chains in the CtIP/Ctp1 tetramer indicates that an ability to bridge DNA ends might be an important feature of CtIP/Ctp1 function, establishing an intriguing similarity with the known ability of the MRE11-RAD50- NBS1 complex to link DNA ends. Although the exact mechanism of action remains to be elucidated, the remarkable evolutionary conservation of CtIP/Ctp1 tetramerisation clearly points to its crucial role in HR.
Keywords
DNA repair, double strand break, Homologous Recombination, DNA-end resection, CtIP, tetramerisation
Sponsorship
Cancer Research UK (11224)
WELLCOME TRUST (104641/Z/14/Z)
Identifiers
External DOI: https://doi.org/10.1080/19491034.2015.1086050
This record's URL: https://www.repository.cam.ac.uk/handle/1810/250319
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