Show simple item record

dc.contributor.authorHerberg, Sarahen
dc.contributor.authorSimeone, Angelaen
dc.contributor.authorOikawa, Mamien
dc.contributor.authorJullien, Jeromeen
dc.contributor.authorBradshaw, Charlesen
dc.contributor.authorTeperek, Martaen
dc.contributor.authorGurdon, Johnen
dc.contributor.authorMiyamoto, Keien
dc.date.accessioned2015-08-27T12:38:32Z
dc.date.available2015-08-27T12:38:32Z
dc.date.issued2015-09-21en
dc.identifier.citationHerberg et al. Scientific Reports (2015) Vol. 5, Article number 14236. doi: 10.1038/srep14236en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/250376
dc.description.abstractTransposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their developmentally regulated expression has been shown, the mechanisms controlling retrotransposon expression in early embryos are still not well understood. Here, we observe a dynamic expression pattern of retrotransposons with three out of ten examined retrotransposons (1a11, λ-olt 2-1 and xretpos(L)) being transcribed solely during early embryonic development. We also identified a transcript that contains the long terminal repeat (LTR) of λ-olt 2-1 and shows a similar expression pattern to λ-olt 2-1 in early Xenopus embryos. All three retrotransposons are transcribed by RNA polymerase II. Although their expression levels decline during development, the LTRs are marked by histone H3 lysine 4 trimethylation. Furthermore, retrotransposons, especially λ-olt 2-1, are enriched with histone H3 lysine 9 trimethylation (H3K9me3) when their expression is repressed. Overexpression of lysine-specific demethylase 4d removes H3K9me3 marks from Xenopus embryos and inhibits the repression of λ-olt 2-1 after gastrulation. Thus, our study shows that H3K9me3 is important for silencing the developmentally regulated retrotransposon in Xenopus laevis.
dc.description.sponsorshipGurdon laboratory is supported by grants from the Wellcome Trust (RG69899) and MRC to J.B.G
dc.languageEnglishen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleHistone H3 lysine 9 trimethylation is required for suppressing the expression of an embryonically activated retrotransposon in Xenopus laevisen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Nature Publishing Group via http://dx.doi.org/10.1038/srep14236en
prism.number14236en
prism.publicationDate2015en
prism.publicationNameScientific Reportsen
prism.volume5en
dc.rioxxterms.funderMRC
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.projectidRG69899
dc.rioxxterms.projectidJ.B.G
dcterms.dateAccepted2015-08-18en
rioxxterms.versionofrecord10.1038/srep14236en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-09-21en
dc.contributor.orcidJullien, Jerome [0000-0002-7868-0021]
dc.contributor.orcidBradshaw, Charles [0000-0002-3528-458X]
dc.contributor.orcidTeperek, Marta [0000-0001-8520-5598]
dc.contributor.orcidGurdon, John [0000-0002-5621-3799]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (101050/Z/13/Z)
pubs.funder-project-idMRC (MR/K011022/1)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales