Modeling mouse and human development using organoid cultures
Company of Biologists
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Huch Ortega, M., & Koo, B. (2015). Modeling mouse and human development using organoid cultures. Development, 142 3113-3125. https://doi.org/10.1242/dev.118570
In vitro 3D cultures are emerging as novel systems with which to study tissue development, organogenesis and stem cell behaviour ex vivo. When grown in a 3D environment, embryonic stem cells (ESCs) self-organize into organoids and acquire the right tissue patterning to develop into several endoderm- and ectoderm-derived tissues, mimicking their in vivo counterparts. Tissue-resident adult stem cells (AdSCs) also form organoids when grown in 3D and can be propagated in vitro for long periods of time. In this review we discuss recent advances in the generation of pluripotent stem cell- and AdSC-derived organoids, highlighting their potential for enhancing our understanding of human development. We will also explore how this new culture system allows disease modeling and gene repair for a personalized regenerative medicine approach.
3D-Organoid Culture, Embryonic Stem Cell, Adult Stem Cell, Disease Modeling, Genetic Engineering
M.H. is a Wellcome Trust Sir Henry Dale Fellow and is jointly funded by the Wellcome Trust and the Royal Society. B.-K.K. is supported by a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society.
Wellcome Trust (101241/Z/13/Z)
WELLCOME TRUST (104151/Z/14/Z)
External DOI: https://doi.org/10.1242/dev.118570
This record's URL: https://www.repository.cam.ac.uk/handle/1810/250464