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dc.contributor.authorManetsberger, Juliaen
dc.contributor.authorHall, Lisaen
dc.contributor.authorChristie, Grahamen
dc.date.accessioned2015-09-03T14:33:56Z
dc.date.available2015-09-03T14:33:56Z
dc.date.issued2015-08-27en
dc.identifier.citationFEMS Microbiology Letters 2015, 362(18): fnv147. doi: 10.1093/femsle/fnv147en
dc.identifier.issn0378-1097
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/250483
dc.description.abstractSpores of Bacillus megaterium QM B1551 are encased in a morphologically distinctive exosporium. We demonstrate here that genes encoded on the indigenous pBM500 and pBM600 plasmids are required for exosporium assembly and or stability in spores of this strain. Bioinformatic analyses identified genes encoding orthologues of the B. cereus-family exosporium nap and basal layer proteins within the B. megaterium genome. Transcriptional analyses, supported by electron and fluorescent microscopy, indicate that the pole-localised nap, identified here for the first time in B. megaterium QM B1551 spores, is comprised of the BclA1 protein. The role of the BxpB protein, which forms the basal layer of the exosporium in B. cereus spores, is less clear since spores of a null mutant strain display an apparently normal morphology. Retention of the localised nap in bxpB null spores suggests that B. megaterium employs an alternative mechanism to that used by B. cereus spores in anchoring the nap to the spore surface.
dc.description.sponsorshipJulia Manetsberger was the recipient of an EPSRC Doctoral Training grant. The assistance of Dr. Jeremy Skepper (University of Cambridge), Caitlin Brumby and Professor Per Bullough (both University of Sheffield) with electron microscopy work is gratefully acknowledged. The authors have no conflicts of interest to declare.
dc.languageEnglishen
dc.language.isoenen
dc.publisherOxford University Press
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.subjectbacillusen
dc.subjectsporesen
dc.subjectexosporiumen
dc.subjectindigenous plasmidsen
dc.subjecthairy napen
dc.titlePlasmid-encoded genes influence exosporium assembly and morphology in Bacillus megaterium QM B1551 sporesen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Oxford University Press via http://dx.doi.org/10.1093/femsle/fnv147en
prism.numberfnv147en
prism.publicationDate2015en
prism.publicationNameFEMS Microbiology Lettersen
prism.volume362en
dc.rioxxterms.funderEPSRC
dcterms.dateAccepted2015-08-24en
rioxxterms.versionofrecord10.1093/femsle/fnv147en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-08-27en
dc.contributor.orcidHall, Lisa [0000-0001-9572-9854]
dc.contributor.orcidChristie, Graham [0000-0001-7177-9646]
dc.identifier.eissn1574-6968
rioxxterms.typeJournal Article/Reviewen


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales