Research data supporting "Systematic E2 screening reveals a UBE2D–RNF138–CtIP axis promoting DNA repair"
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Galanty, Yaron
Sczaniecka-Clift, Matylda
Coates, Julia
Jhujh, Satpal
Description
Complete set of graphs for gammaH2AX, 53BP1 and ubiquitin (FK2 antibody) foci/cell kinetics in response to ionising radiation (IR)- and mock-treated human U2OS cells treated with siRNAs targeting ubiquitin E2s (screening module 1; refers to Fig. 1). Complete set of DDR signalling immunoblots, generated from whole cell extracts of IR-, camptothecin- or mock-treated human U2OS cells treated with siRNAs targeting ubiquitin E2s in screening module 3 (refers to Fig. 3 and Supplementary Fig. 2).
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Keywords
Foci/ cell kinetics after IR, DNA repair, DNA damage response, DNA damage signalling, siRNA screen, ubiquitin, E2 enzymes
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This work was supported by the European Community Seventh
Framework Programme [grant number HEALTH-F2-2010-259893], the European Research Council (DDREAM), CRUK [grant number C6946/A14492], Wellcome Trust [grant number WT092096], the German Research Foundation (DFG) [BE 5342/1-1], and the Return-to-Europe Fellowship (FEBS).