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dc.contributor.authorHarpel, Desireeen
dc.contributor.authorCullen, Darronen
dc.contributor.authorOtt, Swidbert Ren
dc.contributor.authorJiggins, Chrisen
dc.contributor.authorWalters, James Ren
dc.date.accessioned2015-09-07T10:09:27Z
dc.date.available2015-09-07T10:09:27Z
dc.date.issued2015-05-08en
dc.identifier.citationInsect Biochemistry and Molecular Biology 2015, 63, 7-13 doi:10.1016/j.ibmb.2015.04.004en
dc.identifier.issn0965-1748
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/250513
dc.description.abstractWhile most adult Lepidoptera use flower nectar as their primary food source, butterflies in the genus Heliconius have evolved the novel ability to acquire amino acids from consuming pollen. Heliconius butterflies collect pollen on their proboscis, moisten the pollen with saliva, and use a combination of mechanical disruption and chemical degradation to release free amino acids that are subsequently re-ingested in the saliva. Little is known about the molecular mechanisms of this complex pollen feeding adaptation. Here we report an initial shotgun proteomic analysis of saliva from Heliconius melpomene. Results from liquid-chromatography tandem mass-spectrometry confidently identified 31 salivary proteins, most of which contained predicted signal peptides, consistent with extracellular secretion. Further bioinformatic annotation of these salivary proteins indicated the presence of four distinct functional classes: proteolysis (10 proteins), carbohydrate hydrolysis (5), immunity (6), and “housekeeping” (4). Additionally, six proteins could not be functionally annotated beyond containing a predicted signal sequence. The presence of several salivary proteases is consistent with previous demonstrations that Heliconius saliva has proteolytic capacity. It is likely that these proteins play a key role in generating free amino acids during pollen digestion. The identification of proteins functioning in carbohydrate hydrolysis is consistent with Heliconius butterflies consuming nectar, like other lepidopterans, as well as pollen. Immune-related proteins in saliva are also expected, given that ingestion of pathogens is a likely route to infection. The few “housekeeping” proteins are likely not true salivary proteins and reflect a modest level of contamination that occurred during saliva collection. Among the unannotated proteins were two sets of paralogs, each seemingly the result of a relatively recent tandem duplication. These results offer a first glimpse into the molecular foundation of Heliconius pollen feeding and provide a substantial advance towards comprehensively understanding this striking evolutionary novelty.
dc.description.sponsorshipThis work was supported by the Balfour-Browne Fund administered by the Department of Zoology at the University of Cambridge. Additional support came from the University of Kansas.
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/uk/*
dc.subjectSalivaen
dc.subjectProteomicsen
dc.subjectHeliconiusen
dc.subjectPollen feedingen
dc.subjectLepidopteraen
dc.titlePollen feeding proteomics: Salivary proteins of the passion flower butterfly, Heliconius melpomeneen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.ibmb.2015.04.004en
prism.endingPage13
prism.publicationDate2015en
prism.publicationNameInsect Biochemistry and Molecular Biologyen
prism.startingPage7
prism.volume63en
dcterms.dateAccepted2015-04-16en
rioxxterms.versionofrecord10.1016/j.ibmb.2015.04.004en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-05-08en
dc.contributor.orcidCullen, Darron [0000-0002-6287-5086]
dc.contributor.orcidJiggins, Chris [0000-0002-7809-062X]
dc.identifier.eissn1879-0240
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2016-05-08


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Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivs 2.0 UK: England & Wales