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dc.contributor.authorHirst, Jenniferen
dc.contributor.authorEdgar, Jamesen
dc.contributor.authorBorner, Georg HHen
dc.contributor.authorLi, Samen
dc.contributor.authorSahlender, Daniela Aen
dc.contributor.authorAntrobus, Robinen
dc.contributor.authorRobinson, Margareten
dc.date.accessioned2015-09-08T10:07:55Z
dc.date.available2015-09-08T10:07:55Z
dc.date.issued2015-07-15en
dc.identifier.citationMolecular Biology of the Cell 2015, 26(17), 3085-3103. doi: 10.1091/mbc.E15-04-0245en
dc.identifier.issn1059-1524
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/250528
dc.description.abstractThe precise functions of most of the proteins that participate in clathrin-mediated intracellular trafficking are unknown. We have investigated two such proteins, epsinR and gadkin, using the knocksideways method, which rapidly depletes proteins from the available pool by trapping them onto mitochondria. Although epsinR is known to be a SNARE-specific adaptor, the epsinR knocksideways blocked the production of the entire population of intracellular clathrin-coated vesicles (CCVs), suggesting a more global function. Using the epsinR knocksideways data, we were able to estimate the copy number of all major intracellular CCV proteins. Both sides of the vesicle are densely covered, indicating that CCVs sort their cargo by molecular crowding. Trapping of gadkin onto mitochondria also blocked the production of intracellular CCVs, but by a different mechanism: vesicles became crosslinked to mitochondria and pulled out toward the cell periphery. Both phenotypes provide new insights into the regulation of intracellular CCV formation, which could not have been found using more conventional approaches.
dc.languageEnglishen
dc.language.isoenen
dc.publisherThe American Society for Cell Biology
dc.rightsAttribution-NonCommercial-ShareAlike 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.0/uk/*
dc.titleContributions of EpsinR and Gadkin to Clathrin-mediated Intracellular Traffickingen
dc.title.alternativeEpsinR, gadkin, and clathrinen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from The American Society for Cell Biology via http://dx.doi.org/10.1091/mbc.E15-04-0245en
prism.endingPage3103
prism.publicationDate2015en
prism.publicationNameMolecular Biology of the Cellen
prism.startingPage3085
prism.volume26en
dc.rioxxterms.funderWellcome Trust
dcterms.dateAccepted2015-07-06en
rioxxterms.versionofrecord10.1091/mbc.E15-04-0245en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-07-15en
dc.contributor.orcidHirst, Jennifer [0000-0001-9063-8494]
dc.contributor.orcidEdgar, James [0000-0001-7903-8199]
dc.contributor.orcidRobinson, Margaret [0000-0003-0631-0053]
dc.identifier.eissn1939-4586
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (086598/Z/08/Z)
pubs.funder-project-idWellcome Trust (100140/Z/12/Z)
rioxxterms.freetoread.startdate2015-09-15


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Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-ShareAlike 2.0 UK: England & Wales