An orthogonal biocatalytic approach for the safe generation and use of HCN in a multistep continuous preparation of chiral O-acetylcyanohydrins
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Authors
Abstract
An enantioselective preparation of O-acetylcyanohydrins has been accomplished by a three-step telescoped continuous process. The modular components enabled accurate control of two sequential biotransformations, safe handling of an in situ generated hazardous gas, and in-line stabilization of products. This method proved to be advantageous over the batch protocols in terms of reaction time (40 vs 345 min) and ease of operation, opening up access to reactions which have often been neglected due to safety concerns.
Description
Keywords
Arabidopsis thaliana, novozyme 435, biocatalysis, flow, telescoped, HCN, cyanohydrins
Journal Title
Synlett
Conference Name
Journal ISSN
0936-5214
1437-2096
1437-2096
Volume Title
27
Publisher
Georg Thieme Verlag KG
Publisher DOI
Sponsorship
Engineering and Physical Sciences Research Council (EP/K009494/1)
Engineering and Physical Sciences Research Council (EP/K039520/1)
Engineering and Physical Sciences Research Council (EP/K039520/1)
We gratefully acknowledge the Deutsche Forschungsgemeinschaft (DFG) within the research training group GRK 1166 “Biocatalysis in non-conventional media (BioNoCo)”, and the EPSRC (Award Nos. EP/K009494/1 and EP/K039520/1)