Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.
Conduit, Paul T
Novak, Zsofia A
Weil, Timothy T
Raff, Jordan W
eLife Sciences Publications, Ltd
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Conduit, P. T., Wainman, A., Novak, Z. A., Weil, T. T., & Raff, J. W. (2015). Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.. Elife, 4 (e08483) https://doi.org/10.7554/eLife.08483
Centrosomes have many important functions and comprise a 'mother' and 'daughter' centriole surrounded by pericentriolar material (PCM). The mother centriole recruits and organises the PCM and templates the formation of the daughter centriole. It has been reported that several important Drosophila PCM-organising proteins are recruited to centrioles from the cytosol as part of large cytoplasmic 'S-CAP' complexes that contain the centriole protein Sas-4. In a previous paper (Conduit et al., 2014b) we showed that one of these proteins, Cnn, and another key PCM-organising protein, Spd-2, are recruited around the mother centriole before spreading outwards to form a scaffold that supports mitotic PCM assembly; the recruitment of Cnn and Spd-2 is dependent on another S-CAP protein, Asl. We show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4. Thus, PCM recruitment in fly embryos does not appear to require cytosolic S-CAP complexes.
PTC was supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (105653/Z/14/Z) and by an Issac Newton Trust Research Grant from the University of Cambridge awarded to TTW (RG78799). AW, ZN and JWR were supported by a Senior Investigator Award awarded to JWR and funded by the Wellcome Trust (104575/Z/14/Z). The OMX microscope used in this study is part of the Oxford Micron Advanced Bioimaging Unit supported by a Wellcome Trust Strategic Award (091911).
Wellcome Trust (105653/Z/14/Z)
External DOI: https://doi.org/10.7554/eLife.08483
This record's URL: https://www.repository.cam.ac.uk/handle/1810/251319
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Licence URL: http://creativecommons.org/licenses/by/4.0/