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dc.contributor.authorConduit, Paul T
dc.contributor.authorWainman, Alan
dc.contributor.authorNovak, Zsofia A
dc.contributor.authorWeil, Timothy T
dc.contributor.authorRaff, Jordan W
dc.date.accessioned2015-10-06T13:51:59Z
dc.date.available2015-10-06T13:51:59Z
dc.date.issued2015-11-04
dc.identifier.citationeLife 2015, 4: e08483. doi:10.7554/eLife.08483
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/251319
dc.description.abstractCentrosomes have many important functions and comprise a 'mother' and 'daughter' centriole surrounded by pericentriolar material (PCM). The mother centriole recruits and organises the PCM and templates the formation of the daughter centriole. It has been reported that several important Drosophila PCM-organising proteins are recruited to centrioles from the cytosol as part of large cytoplasmic 'S-CAP' complexes that contain the centriole protein Sas-4. In a previous paper (Conduit et al., 2014b) we showed that one of these proteins, Cnn, and another key PCM-organising protein, Spd-2, are recruited around the mother centriole before spreading outwards to form a scaffold that supports mitotic PCM assembly; the recruitment of Cnn and Spd-2 is dependent on another S-CAP protein, Asl. We show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4. Thus, PCM recruitment in fly embryos does not appear to require cytosolic S-CAP complexes.
dc.description.sponsorshipPTC was supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (105653/Z/14/Z) and by an Issac Newton Trust Research Grant from the University of Cambridge awarded to TTW (RG78799). AW, ZN and JWR were supported by a Senior Investigator Award awarded to JWR and funded by the Wellcome Trust (104575/Z/14/Z). The OMX microscope used in this study is part of the Oxford Micron Advanced Bioimaging Unit supported by a Wellcome Trust Strategic Award (091911).
dc.languageEnglish
dc.language.isoen
dc.publishereLife Sciences Publications, Ltd
dc.rightsCreative Commons Attribution 4.0 International License
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleRe-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from eLife via http://dx.doi.org/10.7554/eLife.08483
prism.numbere08483
prism.publicationDate2015
prism.publicationNameElife
prism.volume4
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.projectid105653/Z/14/Z
dc.rioxxterms.projectid104575/Z/14/Z
dc.rioxxterms.projectid091911
dcterms.dateAccepted2015-10-01
rioxxterms.versionofrecord10.7554/eLife.08483
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-11-04
dc.contributor.orcidConduit, Paul [0000-0002-7822-1191]
dc.contributor.orcidWeil, Timothy [0000-0003-0490-849X]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (105653/Z/14/Z)
cam.issuedOnline2015-11-04


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