Structure-function analysis of the Bacillus megaterium GerUD spore germinant receptor protein
Zhou, Ke Xu
FEMS Microbiology Letters
Oxford University Press
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Christie, G., Gupta, S., Zhou, K. X., & Bailey, D. (2015). Structure-function analysis of the Bacillus megaterium GerUD spore germinant receptor protein. FEMS Microbiology Letters, 362 https://doi.org/10.1093/femsle/fnv210
Germination of Bacillus spores is triggered by the interaction of germinant molecules with specialised receptor proteins localised to the spore inner membrane. Germinant receptors (GRs) are comprised typically of three interacting protein subunits, each of which is essential for receptor function. At least some GRs appear to have a fourth component, referred to as a D-subunit protein. A number of D-subunit proteins were shown previously to be capable of modulating the activity of associated GRs. Here we investigate the topology and structurefunction relationships of the Bacillus megaterium QM B1551 GerUD protein, which is associated with the GerU GR. The presented data demonstrate that GerUD can be subjected to relatively extensive structural modifications while retaining function. Indeed, the presence of either of the two transmembrane spanning domains is sufficient to modulate an efficient GerU-mediated germinative response. The precise function of D-subunit proteins has yet to be established, although they may act as molecular chaperones within the spore innermembrane environment.
Bacillus, spore, germination, receptor protein, germinant receptor, D-subunit
S.G was the recipient of a RA Fisher bursary award from Gonville and Caius College, University of Cambridge. X.Z is the recipient of an EPSRC Doctoral Training Grant.
External DOI: https://doi.org/10.1093/femsle/fnv210
This record's URL: https://www.repository.cam.ac.uk/handle/1810/252431
Creative Commons Attribution 4.0 International License
Licence URL: http://creativecommons.org/licenses/by/4.0/