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dc.contributor.authorChristie, Grahamen
dc.contributor.authorGupta, Srishtien
dc.contributor.authorZhou, Ke Xuen
dc.contributor.authorBailey, Daviden
dc.date.accessioned2015-10-28T12:30:42Z
dc.date.available2015-10-28T12:30:42Z
dc.date.issued2015-10-28en
dc.identifier.citationFEMS Microbiology Letters 2015, 362(24). doi:10.1093/femsle/fnv210en
dc.identifier.issn0378-1097
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252431
dc.description.abstractGermination of Bacillus spores is triggered by the interaction of germinant molecules with specialised receptor proteins localised to the spore inner membrane. Germinant receptors (GRs) are comprised typically of three interacting protein subunits, each of which is essential for receptor function. At least some GRs appear to have a fourth component, referred to as a D-subunit protein. A number of D-subunit proteins were shown previously to be capable of modulating the activity of associated GRs. Here we investigate the topology and structurefunction relationships of the Bacillus megaterium QM B1551 GerUD protein, which is associated with the GerU GR. The presented data demonstrate that GerUD can be subjected to relatively extensive structural modifications while retaining function. Indeed, the presence of either of the two transmembrane spanning domains is sufficient to modulate an efficient GerU-mediated germinative response. The precise function of D-subunit proteins has yet to be established, although they may act as molecular chaperones within the spore innermembrane environment.
dc.description.sponsorshipS.G was the recipient of a RA Fisher bursary award from Gonville and Caius College, University of Cambridge. X.Z is the recipient of an EPSRC Doctoral Training Grant.
dc.languageEnglishen
dc.language.isoenen
dc.publisherOxford University Press
dc.rightsCreative Commons Attribution 4.0 International License
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacillusen
dc.subjectsporeen
dc.subjectgerminationen
dc.subjectreceptor proteinen
dc.subjectgerminant receptoren
dc.subjectD-subuniten
dc.titleStructure-function analysis of the Bacillus megaterium GerUD spore germinant receptor proteinen
dc.typeArticle
dc.description.versionThis is the final version of the article. It was first available from OUP via http://dx.doi.org/10.1093/femsle/fnv210en
prism.publicationDate2015en
prism.publicationNameFEMS Microbiology Lettersen
prism.volume362en
dc.rioxxterms.funderEPSRC
dcterms.dateAccepted2015-10-26en
rioxxterms.versionofrecord10.1093/femsle/fnv210en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-10-28en
dc.contributor.orcidChristie, Graham [0000-0001-7177-9646]
dc.identifier.eissn1574-6968
rioxxterms.typeJournal Article/Reviewen


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Creative Commons Attribution 4.0 International License
Except where otherwise noted, this item's licence is described as Creative Commons Attribution 4.0 International License