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dc.contributor.authorShaw, Greg Len
dc.contributor.authorWhitaker, Hayleyen
dc.contributor.authorCorcoran, Marieen
dc.contributor.authorDunning, Marken
dc.contributor.authorLuxton, Hayleyen
dc.contributor.authorKay, Jonathanen
dc.contributor.authorMassie, Charlesen
dc.contributor.authorMiller, Jodien
dc.contributor.authorLamb, Alastairen
dc.contributor.authorRoss-Adams, Helenen
dc.contributor.authorRussell, Roslinen
dc.contributor.authorNelson, Adam Wen
dc.contributor.authorEldridge, Matthewen
dc.contributor.authorLynch, Andyen
dc.contributor.authorRamos-Montoya, Antonioen
dc.contributor.authorMills, Ian Gen
dc.contributor.authorTaylor, Angela Een
dc.contributor.authorArlt, Wiebkeen
dc.contributor.authorShah, Nimishen
dc.contributor.authorWarren, Anneen
dc.contributor.authorNeal, David Een
dc.date.accessioned2015-11-04T11:51:39Z
dc.date.available2015-11-04T11:51:39Z
dc.date.issued2016-08-01en
dc.identifier.citationEuropean Urology 2015. doi:10.1016/j.eururo.2015.10.042en
dc.identifier.issn1421-993X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252506
dc.description.abstractThe androgen receptor (AR) is the dominant growth factor in prostate cancer (PCa). Therefore, understanding how ARs regulate the human transcriptome is of paramount importance. The early effects of castration on human PCa have not previously been studied 27 patients medically castrated with degarelix 7 d before radical prostatectomy. We used mass spectrometry, immunohistochemistry, and gene expression array (validated by reverse transcription-polymerase chain reaction) to compare resected tumour with matched, controlled, untreated PCa tissue. All patients had levels of serum androgen, with reduced levels of intraprostatic androgen at prostatectomy. We observed differential expression of known androgen-regulated genes (TMPRSS2, KLK3, CAMKK2, FKBP5). We identified 749 genes downregulated and 908 genes upregulated following castration. AR regulation of α-methylacyl-CoA racemase expression and three other genes (FAM129A, RAB27A, and KIAA0101) was confirmed. Upregulation of oestrogen receptor 1 (ESR1) expression was observed in malignant epithelia and was associated with differential expression of ESR1-regulated genes and correlated with proliferation (Ki-67 expression).
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsCreative Commons Attribution 4.0 International License
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAndrogen receptoren
dc.subjectCastrationen
dc.subjectClinical trialen
dc.subjectGene transcriptionen
dc.subjectImmunohistochemistryen
dc.subjectProstate canceren
dc.subjectEstrogen Receptor alphaen
dc.subjectGene Expression Profilingen
dc.subjectGene Expression Regulation, Neoplasticen
dc.subjectHormone Antagonistsen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectMaleen
dc.subjectOligopeptidesen
dc.subjectPreoperative Careen
dc.subjectProstateen
dc.subjectProstatectomyen
dc.subjectProstatic Neoplasmsen
dc.subjectReceptors, Androgenen
dc.subjectSpectrum Analysisen
dc.titleThe Early Effects of Rapid Androgen Deprivation on Human Prostate Cancer.en
dc.typeArticle
prism.endingPage218
prism.issueIdentifier2en
prism.publicationDate2016en
prism.publicationNameEuropean Urologyen
prism.startingPage214
prism.volume70en
dc.rioxxterms.funderNIHR
dc.rioxxterms.funderCRUK
dcterms.dateAccepted2015-10-20en
rioxxterms.versionofrecord10.1016/j.eururo.2015.10.042en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-08-01en
dc.contributor.orcidDunning, Mark [0000-0002-8853-9435]
dc.contributor.orcidMassie, Charles [0000-0003-2314-4843]
dc.contributor.orcidMiller, Jodi [0000-0001-6870-204X]
dc.contributor.orcidEldridge, Matthew [0000-0002-5799-8911]
dc.contributor.orcidLynch, Andy [0000-0002-7876-7338]
dc.contributor.orcidWarren, Anne [0000-0002-1170-7867]
dc.identifier.eissn1873-7560
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MR/L00156X/1)
pubs.funder-project-idTHE PROSTATE CANCER CHARITY (G2012-50)
pubs.funder-project-idPROSTATE CANCER UK (PA14-022)
cam.issuedOnline2015-11-10en


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Creative Commons Attribution 4.0 International License
Except where otherwise noted, this item's licence is described as Creative Commons Attribution 4.0 International License