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dc.contributor.authorDorrell, Richard Gen
dc.contributor.authorHinksman, George Aen
dc.contributor.authorHowe, Christopheren
dc.date.accessioned2015-11-24T16:33:09Z
dc.date.available2015-11-24T16:33:09Z
dc.date.issued2016-01-14en
dc.identifier.citationPlant Molecular Biology 2015, 90(3): 233-247. doi:10.1007/s11103-015-0408-9en
dc.identifier.issn0167-4412
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252726
dc.description.abstractPlastids produce a vast diversity of transcripts. These include mature transcripts containing coding sequences, and their processing precursors, as well as transcripts that lack direct coding functions, such as antisense transcripts. Although plastid transcriptomes have been characterised in many plant species, less is known about the transcripts produced in other plastid lineages. We characterised the transcripts produced in the fucoxanthin-containing plastids of the dinoflagellate alga Karenia mikimotoi. This plastid lineage, acquired through tertiary endosymbiosis, utilises transcript processing pathways that are very different from those found in plants and green algae, including 3’ poly(U) tail addition, and extensive substitutional editing of transcript sequences. We have sequenced the plastid transcriptome of K. mikimotoi, and have detected evidence for divergent evolution of fucoxanthin plastid genome sequences. We have additionally characterised polycistronic and monocistronic transcripts from two plastid loci, psbD-tRNA Met-ycf4 and rpl36-rps13-rps11. We find evidence for a range of transcripts produced from each locus that differ in terms of editing state, 5’ end cleavage position, and poly(U) tail addition. Finally, we identify antisense transcripts in Karenia mikimotoi, which appear to undergo different processing events from the corresponding sense transcripts. Overall, our study provides insights into the diversity of transcripts and processing intermediates found in plastid lineages across the eukaryotes.
dc.description.sponsorshipThis work was supported by a BBSRC doctoral training grant [BB/F017464/1, to RGD], and a British Phycological Society summer undergraduate studentship [to GAH].
dc.languageEnglishen
dc.language.isoenen
dc.publisherSpringer
dc.rightsCreative Commons Attribution 4.0 International License
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectRNA processingen
dc.subjecthaptophytesen
dc.subjecttranscript processingen
dc.subjectendosymbiotic gene transferen
dc.subjectchloroplast evolutionen
dc.titleDiversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoien
dc.typeArticle
dc.description.versionThis is the final version of the article. It was first available from Springer via http://dx.doi.org/10.1007/s11103-015-0408-9en
prism.endingPage247
prism.publicationDate2016en
prism.publicationNamePlant Molecular Biologyen
prism.startingPage233
prism.volume90en
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectidBB/F017464/1
dcterms.dateAccepted2015-11-12en
rioxxterms.versionofrecord10.1007/s11103-015-0408-9en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-01-14en
dc.contributor.orcidHowe, Christopher [0000-0002-6975-8640]
dc.identifier.eissn1573-5028
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/F017464/1)


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Except where otherwise noted, this item's licence is described as Creative Commons Attribution 4.0 International License