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dc.contributor.authorMacaulay, Iain Cen
dc.contributor.authorSvensson, Valentineen
dc.contributor.authorLabalette, Charlotteen
dc.contributor.authorFerreira, Laurenen
dc.contributor.authorHamey, Fionaen
dc.contributor.authorVoet, Thierryen
dc.contributor.authorTeichmann, Sarahen
dc.contributor.authorCvejic, Anaen
dc.date.accessioned2015-12-02T11:57:16Z
dc.date.available2015-12-02T11:57:16Z
dc.date.issued2016-01-21en
dc.identifier.citationMacaulay et al. Cell Reports (2016) Vol. 14, Issue 4, pp. 966-977. doi: 10.1016/j.celrep.2015.12.082en
dc.identifier.issn2211-1247
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252796
dc.description.abstractThe transcriptional programs that govern haematopoiesis have mainly been investigated by population-level analysis of haematopoietic stem and progenitor cells, which cannot reveal the continuous nature of the differentiation process. Here we applied single cell RNA-sequencing to a population of haematopoietic cells in zebrafish as they undergo thrombocyte lineage commitment. By reconstructing their developmental chronology computationally, we were able to place each cell along a continuum from stem cell to mature cell, refining the traditional lineage tree. The progression of cells along this continuum is characterised by a highly coordinated transcriptional program, displaying simultaneous suppression of genes involved in cell proliferation and ribosomal biogenesis as the expression of lineage specific genes increases. Within this program, there is substantial heterogeneity in the expression of the key lineage regulators. Overall, the total number of genes expressed, as well as the total mRNA content of the cell, decreases as the cells undergo lineage commitment.
dc.description.sponsorshipThe study was supported by Cancer Research UK grant number C45041/A14953 to A.C., C.L. and L.F and a core support grant from the Wellcome Trust and MRC to the Wellcome Trust–Medical Research Council Cambridge Stem Cell Institute. S.T would like to acknowledge the Lister Research Prize from the Lister Institute. The authors declare no competing financial interests
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier (Cell Press)
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleSingle cell RNA-sequencing reveals a continuous spectrum of differentiation in haematopoietic cellsen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Cell Press via http://dx.doi.org/10.1016/j.celrep.2015.12.082en
prism.endingPage977
prism.publicationDate2016en
prism.publicationNameCell Reportsen
prism.startingPage966
prism.volume14en
dc.rioxxterms.funderCRUK
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderMRC
dc.rioxxterms.projectidC45041/A14953
dcterms.dateAccepted2015-12-16en
rioxxterms.versionofrecord10.1016/j.celrep.2015.12.082en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-01-21en
dc.contributor.orcidHamey, Fiona [0000-0001-7299-2860]
dc.contributor.orcidTeichmann, Sarah [0000-0002-6294-6366]
dc.contributor.orcidCvejic, Ana [0000-0003-3204-9311]
dc.identifier.eissn2211-1247
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_PC_12009)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales